Detail publikace

Isolation, detection and quantification of Hepatitis B Virus using magnetic nanobeads and chip electrophoresis

KUKAČKA, J. HÚSKA, D. POUCH, M. KŘÍŽKOVÁ, S. DRBOHLAVOVÁ, J. CHOMOUCKÁ, J. ADAM, V. HUBÁLEK, J. KLAPKOVÁ, E. PRŮŠA, R. KIZEK, R.

Originální název

Isolation, detection and quantification of Hepatitis B Virus using magnetic nanobeads and chip electrophoresis

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

In this study, magnetic nanobeads coated with streptavidine were prepared to isolate ssDNA specific to Hepatitis B virus (HBV). DNA probes labeled by biotine molecules were designed to be complementary to specific HBV genes. Artificial synthetic oligonucleotides (25–35 nt), identical to HBV gene sequences, were used to mimic a real sample of human blood serum. The amount of nanoparticles utilized for isolation target molecules was within the range from 1 to 20 uL. It was found that already 1 uL of MNPs was sufficient for effective isolation of the molecule of the interest.

Klíčová slova

magnetic nanobeads, chip electrophoresis

Autoři

KUKAČKA, J.; HÚSKA, D.; POUCH, M.; KŘÍŽKOVÁ, S.; DRBOHLAVOVÁ, J.; CHOMOUCKÁ, J.; ADAM, V.; HUBÁLEK, J.; KLAPKOVÁ, E.; PRŮŠA, R.; KIZEK, R.

Vydáno

1. 4. 2011

Nakladatel

FEDERATION AMER SOC EXP BIOL

Místo

BETHESDA, MD 20814-3998 USA

ISSN

1530-6860

Periodikum

FASEB Journal

Ročník

25

Stát

Spojené státy americké

BibTex

@misc{BUT105322,
  author="Jiří {Kukačka} and Dalibor {Húska} and Milan {Pouch} and Soňa {Křížková} and Jana {Drbohlavová} and Jana {Pekárková} and Vojtěch {Adam} and Jaromír {Hubálek} and Eva {Klapková} and Richard {Průša} and René {Kizek}",
  title="Isolation, detection and quantification of Hepatitis B Virus using magnetic nanobeads and chip electrophoresis",
  year="2011",
  journal="FASEB Journal",
  volume="25",
  publisher="FEDERATION AMER SOC EXP BIOL",
  address="BETHESDA, MD 20814-3998 USA",
  issn="1530-6860",
  note="abstract"
}