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MICHALICOVÁ, P. BORŮVKOVÁ, T. MRAVEC, F. ČÁSLAVSKÝ, J. PEKAŘ, M.
Originální název
The use of gas chromatography for determination of residual tert-butanol in dried systems of hyaluronana and fluorescence probes
Typ
abstrakt
Jazyk
angličtina
Originální abstrakt
Hyaluronan is a native biopolymer with hydrophilic character, which contains on its chain also hydrophobic areas. Due to the prevalent hydrophilicity these parts are protected by organized water molecules. For accessing the hydrophobic parts for interactions with hydrophobic fluorescence probes the method of drying was used. During the preparation of samples for drying tert-butanol was added. The problem arise if residual part of tert-butanol remains in the sample due to its hydrophobic organic character, which can significantly affect the result of fluorescence measurement. The amount of residual tert-butanol was determined by head-space extraction with gas chromatography detection.
Klíčová slova
hyaluronan, drying, tert-butanol, gas chromatography
Autoři
MICHALICOVÁ, P.; BORŮVKOVÁ, T.; MRAVEC, F.; ČÁSLAVSKÝ, J.; PEKAŘ, M.
Vydáno
7. 9. 2014
Nakladatel
Czech Chemical Society
ISSN
0009-2770
Periodikum
Chemické listy
Ročník
108
Číslo
8
Stát
Česká republika
Strany od
772
Strany do
Strany počet
1
BibTex
@misc{BUT109297, author="Petra {Michalicová} and Tereza {Borůvková} and Filip {Mravec} and Josef {Čáslavský} and Miloslav {Pekař}", title="The use of gas chromatography for determination of residual tert-butanol in dried systems of hyaluronana and fluorescence probes", booktitle="Chemické listy", year="2014", journal="Chemické listy", volume="108", number="8", pages="772--772", publisher="Czech Chemical Society", issn="0009-2770", note="abstract" }