Detail publikace

Use of green fluorescent proteins for in vitro biosensing.

HEGER, Z. ZÍTKA, O. FOHLEROVÁ, Z. MERLOS RODRIGO, M. HUBÁLEK, J. KIZEK, R. ADAM, V.

Originální název

Use of green fluorescent proteins for in vitro biosensing.

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Due to the considerable stability of green fluorescent proteins and their capacity to be readily permutated or mutated, they may be exploited in multiple ways to enhance the functionality of in vitro biosensors. Many possibilities, such as the formation of chimeras with other proteins or antibodies, as well as Förster resonance emission transfer performance, may be used for the highly sensitive and specific detection of the target molecules. This review considers the great potential of green fluorescent proteins as the fluorescent probing or recognition biomolecule in various in vitro biosensors applications, as well as obstacles associated with their use.

Klíčová slova

Aequorea victoria; biosensor; chimera; emission; Förster resonance emission transfer

Autoři

HEGER, Z.; ZÍTKA, O.; FOHLEROVÁ, Z.; MERLOS RODRIGO, M.; HUBÁLEK, J.; KIZEK, R.; ADAM, V.

Rok RIV

2015

Vydáno

1. 1. 2015

Nakladatel

Chemical Papers

ISSN

0366-6352

Periodikum

Chemical Papers

Ročník

69

Číslo

1

Stát

Slovenská republika

Strany od

54

Strany do

61

Strany počet

8

BibTex

@article{BUT112066,
  author="Zbyněk {Heger} and Ondřej {Zítka} and Zdenka {Fohlerová} and Miguel Ángel {Merlos Rodrigo} and Jaromír {Hubálek} and René {Kizek} and Vojtěch {Adam}",
  title="Use of green fluorescent proteins for in vitro biosensing.",
  journal="Chemical Papers",
  year="2015",
  volume="69",
  number="1",
  pages="54--61",
  doi="10.2478/s11696-014-0588-9",
  issn="0366-6352"
}