Detail publikace

Automated alignment method for coherence-controlled holographic microscope

DOSTÁL, Z. SLABÝ, T. KVASNICA, L. LOŠŤÁK, M. KŘÍŽOVÁ, A. CHMELÍK, R.

Originální název

Automated alignment method for coherence-controlled holographic microscope

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Coherence-controlled holographic microscope (CCHM) was developed particularly for quantitative phase imaging and measurement of live cell dynamics, which is the proper subject of digital holographic microscopy (DHM). CCHM in low-coherence mode extends DHM in the study of living cells. However, this advantage is compensated by sensitivity of the system to easily become misaligned, which is a serious hindrance to wanted performance. Therefore, it became clear that introduction of a self-correcting system is inevitable. Accordingly, we had to devise a theory of a suitable control and design an automated alignment system for CCHM. The modulus of the reconstructed holographic signal was identified as a significant variable for guiding the alignment procedures. From this, we derived the original basic realignment three-dimensional algorithm, which encompasses a unique set of procedures for automated alignment that contains processes for initial and advanced alignment as well as long-term maintenance of microscope tuning. All of these procedures were applied to a functioning microscope and the tested processes were successfully validated. Finally, in such a way, CCHM is enabled to substantially contribute to study of biology, particularly of cancer cells in vitro.

Klíčová slova

holographic microscopy; quantitative phase imaging; automated alignment; holographic signal

Autoři

DOSTÁL, Z.; SLABÝ, T.; KVASNICA, L.; LOŠŤÁK, M.; KŘÍŽOVÁ, A.; CHMELÍK, R.

Rok RIV

2015

Vydáno

28. 10. 2015

Nakladatel

SPIE

ISSN

1083-3668

Periodikum

JOURNAL OF BIOMEDICAL OPTICS

Ročník

20

Číslo

11

Stát

Spojené státy americké

Strany od

1

Strany do

8

Strany počet

8

URL

https://www.spiedigitallibrary.org/journals/Journal-of-Biomedical-Optics/volume-20/issue-11/111215/Automated-alignment-method-for-coherence-controlled-holographic-microscope/10.1117/1.JBO.20.11.111215.full

Plný text v Digitální knihovně

http://hdl.handle.net/11012/194979

BibTex

@article{BUT117913,
  author="Zbyněk {Dostál} and Tomáš {Slabý} and Lukáš {Kvasnica} and Martin {Lošťák} and Aneta {Křížová} and Radim {Chmelík}",
  title="Automated alignment method for coherence-controlled holographic microscope",
  journal="JOURNAL OF BIOMEDICAL OPTICS",
  year="2015",
  volume="20",
  number="11",
  pages="1--8",
  doi="10.1117/1.JBO.20.11.111215",
  issn="1083-3668",
  url="https://www.spiedigitallibrary.org/journals/Journal-of-Biomedical-Optics/volume-20/issue-11/111215/Automated-alignment-method-for-coherence-controlled-holographic-microscope/10.1117/1.JBO.20.11.111215.full"
}