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NEDĚLA, V. HŘIB, J. HAVEL, L. HUDEC, J. RUNŠTUK, J.
Originální název
Imaging of Norway spruce early somatic embryos with the ESEM, Cryo-SEM and laser scanning microscope
Typ
článek v časopise - ostatní, Jost
Jazyk
angličtina
Originální abstrakt
This article describes the surface structure of Norway spruce early somatic embryos (ESEs) as a typical culture with asynchronous development. The microstructure of extracellular matrix covering ESEs were observed using the environmental scanning electron microscope as a primary tool and using the scanning electron microscope with cryo attachment and laser electron microscope as a complementary tool allowing our results to be proven independently. The fresh samples were observed in conditions of the air environment of the environmental scanning electron microscope (ESEM) with the pressure from 550 Pa to 690 Pa and the low temperature of the sample from −18 °C to −22 °C. The samples were studied using two different types of detector to allow studying either the thin surface structure or material composition. The scanning electron microscope with cryo attachment was used for imaging frozen extracellular matrix microstructure with higher resolution. The combination of both electron microscopy methods was suitable for observation of “native” plant samples, allowing correct evaluation of our results, free of error and artifacts.
Klíčová slova
ESEM; Cryo-SEM; Bright field/dark field microscopy; Extracellular matrix; Picea abies; Somatic embryogenesis
Autoři
NEDĚLA, V.; HŘIB, J.; HAVEL, L.; HUDEC, J.; RUNŠTUK, J.
Vydáno
2. 3. 2016
Nakladatel
Elsevier
ISSN
0968-4328
Periodikum
Micron
Ročník
2016
Číslo
84
Stát
Spojené království Velké Británie a Severního Irska
Strany od
67
Strany do
71
Strany počet
5
URL
http://www.sciencedirect.com/science/article/pii/S0968432816300208
BibTex
@article{BUT123955, author="Vilém {Neděla} and Jiří {Hřib} and Ladislav {Havel} and Jiří {Hudec} and Jiří {Runštuk}", title="Imaging of Norway spruce early somatic embryos with the ESEM, Cryo-SEM and laser scanning microscope", journal="Micron", year="2016", volume="2016", number="84", pages="67--71", doi="10.1016/j.micron.2016.02.011", issn="0968-4328", url="http://www.sciencedirect.com/science/article/pii/S0968432816300208" }