Detail publikace

Turbidimetry and Fluorescence Study of Effect of Hyaluronan Addition to Cationic Micellar Solution

PILGROVÁ, T. PEKAŘ, M.

Originální název

Turbidimetry and Fluorescence Study of Effect of Hyaluronan Addition to Cationic Micellar Solution

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

Hyaluronan as negatively charged polyelectrolyte can interact with positively charged surfactant micelles via electrostatic interactions to form core-shell like aggregates. These self-assembly systems can solubilize hydrophobic active substances and therefore, they are potential carriers in drug delivery applications. This study deals with electrostatic association of cationic micelles of CTAB or Septonex with hyaluronan. The cationic micelle/hyaluronan complexes were studied using turbidimetry and fluorescence spectroscopy method. Turbidimetric titration was chosen as an indicator of the loss of transmitted light intensity due to the scattering effect of particles associated from hyaluronan and surfactant in it. Fluorescent probe pyrene was selected for spectroscopy experiments because of its unique sensitivity to polarity of the medium, so this fluorescence method was used as an indicator of presence of hydrophobic domains in the system. Effect of components concentration and molecular weight of hyaluronan were evaluated. Results of turbidimetry revealed that aggregates formation (turbidity increasing) depends especially on a charge ratio of surfactant molecules and hyaluronan carboxyl groups. It was found a difference in association of low molecular weight and high molecular weight of hyaluronan. Fluorescence results confirmed presence and stability of micellar aggregates in studied systems.

Klíčová slova

hyaluronan, surfactant, turbidimetry, fluorescence, pyrene

Autoři

PILGROVÁ, T.; PEKAŘ, M.

Vydáno

11. 9. 2016

Nakladatel

Leibniz-Institut für Polymerforschung Dresden e. V.

Místo

Dresden, Germany

ISBN

978-3-9816007-4-2

Kniha

Book of Abstracts, 20th European Symposium on Polymer Spectroscopy (ESOPS)

Strany od

64

Strany do

64

Strany počet

1

URL

http://www.ipfdd.de/fileadmin/user_upload/oea/00_Tagungsband_ESOPS20_2016-09-15.pdf

BibTex

@misc{BUT128282,
  author="Tereza {Pilgrová} and Miloslav {Pekař}",
  title="Turbidimetry and Fluorescence Study of Effect of Hyaluronan Addition to Cationic Micellar Solution",
  booktitle="Book of Abstracts,  20th European Symposium on Polymer Spectroscopy (ESOPS)",
  year="2016",
  pages="1",
  publisher="Leibniz-Institut für Polymerforschung Dresden e. V.",
  address="Dresden, Germany",
  isbn="978-3-9816007-4-2",
  url="http://www.ipfdd.de/fileadmin/user_upload/oea/00_Tagungsband_ESOPS20_2016-09-15.pdf",
  note="abstract"
}