Detail publikace

Superficial cells are self-renewing chondrocyte progenitors, which form the articular cartilage in juvenile mice

Li, L., Newton, P. T., Bouderlique, T., Sejnohova, M., Zikmund, T., Kozhemyakina, E., Xie, M., Krivanek, J., Kaiser, J., Qian, H., Dyachuk, V., Lassar, A. B., Warman, M. L., Barenius, B., Adameyko, I., Chagin, A. S.

Originální název

Superficial cells are self-renewing chondrocyte progenitors, which form the articular cartilage in juvenile mice

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Articular cartilage has little regenerative capacity. Recently, genetic lineage tracing experiments have revealed chondrocyte progenitors at the articular surface. We further characterized these progenitors by using in vivo genetic approaches. Histone H2B–green fluorescent protein retention revealed that superficial cells divide more slowly than underlying articular chondrocytes. Clonal genetic tracing combined with immunohistochemistry revealed that superficial cells renew their number by symmetric division, express mesenchymal stem cell markers, and generate chondrocytes via both asymmetric and symmetric differentiation. Quantitative analysis of cellular kinetics, in combination with phosphotungstic acid–enhanced micro–computed tomography,showed that superficial cells generate chondrocytes and contribute to the growth and reshaping of articular cartilage. Furthermore, we found that cartilage renewal occurs as the progeny of superficial cells fully replace fetal chondrocytes during early postnatal life. Thus, superficial cells are self-renewing progenitors that are capable of maintaining their own population and fulfilling criteria of unipotent adult stem cells. Furthermore, the progeny of these cells reconstitute adult articular cartilage de novo, entirely substituting fetal chondrocytes.

Klíčová slova

superficial zone, bone, adult stem cells, regeneration, osteoarthritis

Autoři

Li, L., Newton, P. T., Bouderlique, T., Sejnohova, M., Zikmund, T., Kozhemyakina, E., Xie, M., Krivanek, J., Kaiser, J., Qian, H., Dyachuk, V., Lassar, A. B., Warman, M. L., Barenius, B., Adameyko, I., Chagin, A. S.

Vydáno

1. 3. 2017

ISSN

0892-6638

Periodikum

The FASEB Journal

Ročník

31

Číslo

3

Stát

Spojené státy americké

Strany od

1067

Strany do

1084

Strany počet

18

BibTex

@article{BUT130706,
  author="Jozef {Kaiser} and Tomáš {Zikmund} and Marie {Šejnohová}",
  title="Superficial cells are self-renewing chondrocyte progenitors, which form the articular cartilage in juvenile mice",
  journal="The FASEB Journal",
  year="2017",
  volume="31",
  number="3",
  pages="1067--1084",
  doi="10.1096/fj.201600918R",
  issn="0892-6638"
}