Přístupnostní navigace
E-přihláška
Vyhledávání Vyhledat Zavřít
Detail publikace
ADAMCOVÁ, Z. MRAVEC, F. PEKAŘ, M.
Originální název
Determination and Comparision of Micelle Radius of Different Surfactants Using Förster Resonance Energy Transfer
Typ
abstrakt
Jazyk
angličtina
Originální abstrakt
This contribution is focused on utilization of Förster resonance energy transfer as a nanoscale ruler in order to determine micellar radii of three cationic surfactants with different hydrophobic chain length – CTAB (C16), Septonex (C15) and TTAB (C14). As a convenient donor-acceptor pair, Perylene (which is solubilized inside micelles) and Fluorescein (situated in the outer hydrophilic part of micelles) were chosen. Located in close proximity (nm), Fluorescein quenches fluorescence of Perylene, the effectivity of which is in direct relation to their distance – radius of micelle in this case. Results obtained by FRET were compared with FCS (Fluorescence Correlation Spectroscopy) measurements, which determine micelle radius on the basis of its diffusion coefficient. In this experiment micelles were labeled only with Perylene in order to avoid effects of fluorescent labeling on the surface of micelles.
Klíčová slova
FRET, perylene, fluorescein, cationic micelles
Autoři
ADAMCOVÁ, Z.; MRAVEC, F.; PEKAŘ, M.
Vydáno
30. 5. 2017
Nakladatel
Masaryk University
Místo
Brno
ISBN
978-80-210-8580-0
Kniha
Book of abstracts
Číslo edice
1
Strany od
46
Strany do
Strany počet
84
BibTex
@misc{BUT138433, author="Zuzana {Adamcová} and Filip {Mravec} and Miloslav {Pekař}", title="Determination and Comparision of Micelle Radius of Different Surfactants Using Förster Resonance Energy Transfer", booktitle="Book of abstracts", year="2017", edition="1", pages="46--46", publisher="Masaryk University", address="Brno", isbn="978-80-210-8580-0", note="abstract" }