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KOŠČOVÁ, P. SEDLÁŘ, K. KUPKOVÁ, K. KOLEK, J. PATÁKOVÁ, P. PROVAZNÍK, I.
Originální název
TERTIARY STRUCTURE PREDICTION OF POTENTIAL EFFLUX-PUMP PROTEIN IN CLOSTRIDIUM BEBERINCKII NRRL B-598
Typ
článek ve sborníku ve WoS nebo Scopus
Jazyk
angličtina
Originální abstrakt
The fields of computational biochemistry and structural biology are growing rapidly. With various computational tools the whole workflow for in silico modeling of three-dimensional protein structures can be compiled. Here, we present a method for tertiary structure prediction for arcB1, a putative efflux pump gene, recently identified in the genomic sequence of Clostridium beijerinckii NRRL B-598. The strain C. beijerinckii NRRL B-598, formerly misidentified as C. pasteurianum, is an oxygen tolerant heterofermentative anaerobe with the ability of acetone-butanol fermentation, with butanol as the main product. Unfortunately, the mechanism of butanol efflux remains unknown. The translated AcrB1 protein sequence had a high similarity to the already described efflux-pump of Escherichia coli. To study these sequences in a structural context, we constructed a homology model for C. beijerinckii NRRL B-598 and compared it with the corresponding experimentally determined structure from E. coli. The predicted protein model helps us to understand its function and confirm that acrB1 is a gene for nonspecific efflux pump — a potential pump capable of butanol efflux.
Klíčová slova
Clostridium; ABE fermentation; efflux pump
Autoři
KOŠČOVÁ, P.; SEDLÁŘ, K.; KUPKOVÁ, K.; KOLEK, J.; PATÁKOVÁ, P.; PROVAZNÍK, I.
Vydáno
31. 10. 2017
Nakladatel
Ocean Design
Místo
Praha
ISBN
978-80-86238-62-3
Kniha
Proceedings of the 5th International Conference on Chemical Technology
Edice
1
Číslo edice
Strany od
26
Strany do
30
Strany počet
5
URL
http://www.icct.cz/AngiologyKlon-ICCT/media/system/ICCT2017-full_papers.pdf
BibTex
@inproceedings{BUT141676, author="Pavlína {Cicková} and Karel {Sedlář} and Kristýna {Kupková} and Jan {Kolek} and Petra {Patáková} and Valentine {Provazník}", title="TERTIARY STRUCTURE PREDICTION OF POTENTIAL EFFLUX-PUMP PROTEIN IN CLOSTRIDIUM BEBERINCKII NRRL B-598", booktitle="Proceedings of the 5th International Conference on Chemical Technology", year="2017", series="1", number="1", pages="26--30", publisher="Ocean Design", address="Praha", isbn="978-80-86238-62-3", url="http://www.icct.cz/AngiologyKlon-ICCT/media/system/ICCT2017-full_papers.pdf" }