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LOŠŤÁK, M. ČOLLÁKOVÁ, J. SLABÝ, T. KŘÍŽOVÁ, A. VESELÝ, P. CHMELÍK, R.
Originální název
Coherence-controlled holographic microscopy for live-cell quantitative phase imaging in turbid media
Typ
článek ve sborníku ve WoS nebo Scopus
Jazyk
angličtina
Originální abstrakt
In this work we present the coherence controlled holographic microscopy (CCHM)(1) and its ability to image the living cells in turbid media(2). The CCHM method and its advantages are introduced. A 'coherence gate effect'(3), that enables imaging in turbid media, occurs owing to the low coherence illumination in our setup. The coherence gate effect is briefly theoretically explained and comparison of images with different illumination sources is shown. After that, the possibility of imaging in turbid media is applied to investigation of cell reactions to cytopathic turbid emulsions. In our experiments we used human cancer cells treated by biologically active phospholipids (BAPs). Cellular events leading to cell death, that would otherwise remain hidden in turbid media, are clearly observable and according to them cell fate can be deduced.
Klíčová slova
holographic microscopy; quantitative phase imaging; coherence gate effect; live cells; turbid media; low coherent interferometry; cancer treatment
Autoři
LOŠŤÁK, M.; ČOLLÁKOVÁ, J.; SLABÝ, T.; KŘÍŽOVÁ, A.; VESELÝ, P.; CHMELÍK, R.
Vydáno
9. 3. 2016
ISBN
978-1-62841-952-8
Kniha
QUANTITATIVE PHASE IMAGING II
ISSN
0277-786X
Periodikum
Proceedings of SPIE
Ročník
9718
Stát
Spojené státy americké
Strany od
97181Y-1
Strany do
97181Y-5
Strany počet
5
URL
https://www.spiedigitallibrary.org/conference-proceedings-of-spie/9718/1/Coherence-controlled-holographic-microscopy-for-live-cell-quantitative-phase-imaging/10.1117/12.2209322.short