Detail publikace

Coherence-controlled holographic microscopy for live-cell quantitative phase imaging in turbid media

LOŠŤÁK, M. ČOLLÁKOVÁ, J. SLABÝ, T. KŘÍŽOVÁ, A. VESELÝ, P. CHMELÍK, R.

Originální název

Coherence-controlled holographic microscopy for live-cell quantitative phase imaging in turbid media

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

In this work we present the coherence controlled holographic microscopy (CCHM)(1) and its ability to image the living cells in turbid media(2). The CCHM method and its advantages are introduced. A 'coherence gate effect'(3), that enables imaging in turbid media, occurs owing to the low coherence illumination in our setup. The coherence gate effect is briefly theoretically explained and comparison of images with different illumination sources is shown. After that, the possibility of imaging in turbid media is applied to investigation of cell reactions to cytopathic turbid emulsions. In our experiments we used human cancer cells treated by biologically active phospholipids (BAPs). Cellular events leading to cell death, that would otherwise remain hidden in turbid media, are clearly observable and according to them cell fate can be deduced.

Klíčová slova

holographic microscopy; quantitative phase imaging; coherence gate effect; live cells; turbid media; low coherent interferometry; cancer treatment

Autoři

LOŠŤÁK, M.; ČOLLÁKOVÁ, J.; SLABÝ, T.; KŘÍŽOVÁ, A.; VESELÝ, P.; CHMELÍK, R.

Vydáno

9. 3. 2016

ISBN

978-1-62841-952-8

Kniha

QUANTITATIVE PHASE IMAGING II

ISSN

0277-786X

Periodikum

Proceedings of SPIE

Ročník

9718

Stát

Spojené státy americké

Strany od

97181Y-1

Strany do

97181Y-5

Strany počet

5

URL