Detail publikace

Satellite DNA and Transposable Elements in Seabuckthorn ( Hippophae rhamnoides ), a Dioecious Plant with Small Y and Large X Chromosomes

PUTEROVÁ, J. RAZUMOVA, O. MARTÍNEK, T. ALEXANDROV, O. DIVASHUK, M. KUBÁT, Z. HOBZA, R. KARLOV, G. KEJNOVSKÝ, E.

Originální název

Satellite DNA and Transposable Elements in Seabuckthorn ( Hippophae rhamnoides ), a Dioecious Plant with Small Y and Large X Chromosomes

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Seabuckthorn (Hippophae rhamnoides) is a dioecious shrub commonly used in the pharmaceutical, cosmetic, and environmental industry as a source of oil, minerals and vitamins. In this study, we analyzed the transposable elements and satellites in its genome.We carried out Illumina DNA sequencing and reconstructed the main repetitive DNA sequences. For data analysis, we developed a new bioinformatics approach for advanced satellite DNA analysis and showed that about 25% of the genome consists of satellite DNA and about 24% is formed of transposable elements, dominated by Ty3/Gypsy and Ty1/Copia LTR retrotransposons. FISH mapping revealed X chromosome-accumulated, Y chromosome-specific or both sex chromosomes-accumulated satellites but most satellites were found on autosomes. Transposable elements were located mostly in the subtelomeres of all chromosomes. The 5S rDNA and 45S rDNA were localized on one autosomal locus each. Although we demonstrated the small size of the Y chromosome of the seabuckthorn and accumulated satellite DNA there, we were unable to estimate the age and extent of the Y chromosome degeneration. Analysis of dioecious relatives such as Shepherdia would shed more light on the evolution of these sex chromosomes.

Klíčová slova

sex chromosomes, genome composition, chromosomal localization, repetitive DNA

Autoři

PUTEROVÁ, J.; RAZUMOVA, O.; MARTÍNEK, T.; ALEXANDROV, O.; DIVASHUK, M.; KUBÁT, Z.; HOBZA, R.; KARLOV, G.; KEJNOVSKÝ, E.

Vydáno

2. 2. 2017

ISSN

1759-6653

Periodikum

GENOME BIOL EVOL

Ročník

9

Číslo

1

Stát

Spojené království Velké Británie a Severního Irska

Strany od

197

Strany do

212

Strany počet

16

URL

https://academic.oup.com/gbe/article/9/1/197/2830930

Plný text v Digitální knihovně

http://hdl.handle.net/11012/137198

BibTex

@article{BUT144426,
  author="Janka {Puterová} and Olga {Razumova} and Tomáš {Martínek} and Oleg {Alexandrov} and Mikhail {Divashuk} and Zdeněk {Kubát} and Roman {Hobza} and Gennady {Karlov} and Eduard {Kejnovský}",
  title="Satellite DNA and Transposable Elements in Seabuckthorn ( Hippophae rhamnoides ), a Dioecious Plant with Small Y and Large X Chromosomes",
  journal="GENOME BIOL EVOL",
  year="2017",
  volume="9",
  number="1",
  pages="197--212",
  doi="10.1093/gbe/evw303",
  issn="1759-6653",
  url="https://academic.oup.com/gbe/article/9/1/197/2830930"
}