Detail publikace

FireProt: web server for automated design of thermostable proteins

MUSIL, M. ŠTOURAČ, J. BENDL, J. BREZOVSKÝ, J. PROKOP, Z. ZENDULKA, J. MARTÍNEK, T. BEDNÁŘ, D. DAMBORSKÝ, J.

Originální název

FireProt: web server for automated design of thermostable proteins

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

There is a continuous interest in increasing proteins stability to enhance their usability in numerous biomedical and biotechnological applications. A number of in silico tools for the prediction of the effect of mutations on protein stability have been developed recently. However, only single-point mutations with a small effect on protein stability are typically predicted with the existing tools and have to be followed by laborious protein expression, purification, and characterization. Here, we present FireProt, a web server for the automated design of multiplepoint thermostable mutant proteins that combines structural and evolutionary information in its calculation core. FireProt utilizes sixteen tools and three protein engineering strategies for making reliable protein designs. The server is complemented with interactive, easy-to-use interface that allows users to directly analyze and optionally modify designed thermostable mutants. FireProt is freely available at http://loschmidt.chemi.muni.cz/fireprot.

Klíčová slova

mutations, protein engineering, mutant proteins, protein stability, thermostable proteins

Autoři

MUSIL, M.; ŠTOURAČ, J.; BENDL, J.; BREZOVSKÝ, J.; PROKOP, Z.; ZENDULKA, J.; MARTÍNEK, T.; BEDNÁŘ, D.; DAMBORSKÝ, J.

Vydáno

26. 4. 2017

ISSN

1362-4962

Periodikum

Nucleic Acids Research

Ročník

45

Číslo

1

Stát

Spojené království Velké Británie a Severního Irska

Strany od

393

Strany do

399

Strany počet

7

URL

https://doi.org/10.1093/nar/gkx285

BibTex

@article{BUT144434,
  author="Miloš {Musil} and Jan {Štourač} and Jaroslav {Bendl} and Jan {Brezovský} and Zbyněk {Prokop} and Jaroslav {Zendulka} and Tomáš {Martínek} and David {Bednář} and Jiří {Damborský}",
  title="FireProt: web server for automated design of thermostable proteins",
  journal="Nucleic Acids Research",
  year="2017",
  volume="45",
  number="1",
  pages="393--399",
  doi="10.1093/nar/gkx285",
  issn="1362-4962",
  url="https://doi.org/10.1093/nar/gkx285"
}