Detail publikace

Determination of hydroxyproline using ion-exchange liquid chromatography with vis detector and high performance liquid chromatography with fluorescence detector

LACKOVÁ, Z. CERNEI, N. ŠTĚRBOVÁ, D. HADDAD, Y. ROZÍKOVÁ, V. KOMPRDA, T. ZÍTKA, O.

Originální název

Determination of hydroxyproline using ion-exchange liquid chromatography with vis detector and high performance liquid chromatography with fluorescence detector

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

The first aim of the experiment was an optimization of the method for determination of hydroxyproline in a pig skin using high-performance liquid chromatography with fluorescence detector (HPLC-FLD) and ion-exchange liquid chromatography with VIS detector (IEC-VIS). On the basis of the experiments performed, it was found that HPLC-FLD method is three times more sensitive than IEC-VIS method. For IEC analysis of hydroxyproline, the limit of detection (LOD) was 4.10 μg/ml whereas the limit of quantification (LOQ) was 13.50 μg/ml. For HPLC analysis of hydroxyproline, the limit of detection (LOD) was 1 ng/ml whereas the limit of quantification (LOQ) was 3 ng/ml. The second aim was the testing of influence of different volume of 6M HCl on extraction of sample (50 mg) for analysis using HPLC-FLD. Here it was found that the best volume was 250 µl 6M HCl.

Klíčová slova

Hydroxyproline; HPLC-FLD; IEC-VIS; microwave hydrolysis; pig skin

Autoři

LACKOVÁ, Z.; CERNEI, N.; ŠTĚRBOVÁ, D.; HADDAD, Y.; ROZÍKOVÁ, V.; KOMPRDA, T.; ZÍTKA, O.

Vydáno

31. 12. 2017

Nakladatel

Mendel University in Brno

ISBN

978-80-7509-529-9

Kniha

MendelNet 2017

Strany od

905

Strany do

910

Strany počet

6

URL

https://mendelnet.cz/artkey/mnt-201701-0104_Determination-of-hydroxyproline-using-ion-exchange-liquid-chromatography-with-VIS-detector-and-high-performance.php?back=/magno/mnt/2017/mn1.php?secid=4

Plný text v Digitální knihovně

http://hdl.handle.net/11012/84181

BibTex

@inproceedings{BUT148747,
  author="Zuzana {Lacková} and Natalia Vladimirovna {Cernei} and Dagmar {Štěrbová} and Yazan Abdulmajeed Eyadh {Haddad} and Veronika {Rozíková} and Tomáš {Komprda} and Ondřej {Zítka}",
  title="Determination of hydroxyproline using ion-exchange liquid chromatography with vis detector and high performance liquid chromatography with fluorescence detector",
  booktitle="MendelNet 2017",
  year="2017",
  pages="905--910",
  publisher="Mendel University in Brno",
  isbn="978-80-7509-529-9",
  url="https://mendelnet.cz/artkey/mnt-201701-0104_Determination-of-hydroxyproline-using-ion-exchange-liquid-chromatography-with-VIS-detector-and-high-performance.php?back=/magno/mnt/2017/mn1.php?secid=4"
}