Detail publikace

Separation of Nucleobases Using High-performance Liquid Chromatography Coupled with Voltammetric Scanning

ZÍTKA, O. KUDR, J. LABUDA, J. XHAXHIU, K. ADAM, V.

Originální název

Separation of Nucleobases Using High-performance Liquid Chromatography Coupled with Voltammetric Scanning

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

In this paper, a method based on chromatographic separation of analytes and their quantification using on-line cyclic voltammetry is reported. The method based on high performance liquid chromatography on reverse phase column was optimized using free nucleobases-guanine, adenine, thymine, and cytosine. The optimal separation of nucleobases was detected when using 0.05M borate buffer (pH9.0) as the mobile phase, at isocratic flow rate 1mLmin(-1), and at separation column temperature of 30 degrees C. The accurate timing of the cyclic voltammetry enabled us to quantify the concentrations of individual nucleobases by oxidation on glassy carbon electrode.

Klíčová slova

Glassy carbon electrode; On-line voltammetry scan; Nucleic acid; Nucleobases; Reverse phase

Autoři

ZÍTKA, O.; KUDR, J.; LABUDA, J.; XHAXHIU, K.; ADAM, V.

Vydáno

31. 8. 2018

ISSN

1040-0397

Periodikum

ELECTROANALYSIS

Ročník

30

Číslo

8

Stát

Spolková republika Německo

Strany od

1857

Strany do

1861

Strany počet

5

BibTex

@article{BUT149617,
  author="Ondřej {Zítka} and Jiří {Kudr} and Ján {Labuda} and Kledi {Xhaxhiu} and Vojtěch {Adam}",
  title="Separation of Nucleobases Using High-performance Liquid Chromatography Coupled with Voltammetric Scanning",
  journal="ELECTROANALYSIS",
  year="2018",
  volume="30",
  number="8",
  pages="1857--1861",
  doi="10.1002/elan.201800175",
  issn="1040-0397"
}