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TROSCHL, C. MEIXNER, K. FRITZ, I. LEITNER, K. PALACIOS ROMERO, P. KOVALČÍK, A. SEDLÁČEK, P. DROSG, B.
Originální název
Pilot-scale production of poly-β-hydroxybutyrate with the cyanobacterium Synechocytis sp. CCALA192 in a non-sterile tubular photobioreactor
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
The biopolymer poly-β-hydroxybutyrate (PHB) can be used as a promising bioplastic. It has a broad range of applications and is degraded relatively rapidly by soil organisms. Like many prokaryotes, the cyanobacterium Synechocystis sp. CCALA192 produces this biopolymer as a storage compound, especially under nutrient limitation. In a 200-L tubular photobioreactor, we cultivated Synechocystis sp. CCALA192 semi-continuously over a period of 75 days with CO2 as sole carbon source. A two-stage cultivation strategy was performed, where after 5–7 days nitrogen was depleted and the culture started to produce PHB and gradually turned from blue-green to yellow. After 16–20 days, 90% of the culture were harvested and the residual 10% were used as inoculum for the following cycle. The harvested culture had an average biomass concentration of 1.0 g/L with an average PHB content of 12.5% of cell dry weight. After restarting with fresh nutrients, the yellow culture turned blue-green again and degraded the PHB within 24–48 h. When nitrogen of the medium was consumed, PHB was produced again and the cycle continued. In the late stage of each production cycle, a ripening process was observed, where no CO2 was consumed but the PHB concentration was still rising at the expense of the existing glycogen rich biomass. Establishing a stable Synechocystis sp. CCALA192 culture under non-sterile conditions turned out to be difficult, as this small unicellular organism is very sensitive and easily grazed by protozoa. Therefore, a special cultivation strategy with partially anoxic conditions was necessary.
Klíčová slova
Cyanobacteria; Synechocystis sp. CCALA192; PHB; Tubular photobioreactor
Autoři
TROSCHL, C.; MEIXNER, K.; FRITZ, I.; LEITNER, K.; PALACIOS ROMERO, P.; KOVALČÍK, A.; SEDLÁČEK, P.; DROSG, B.
Vydáno
20. 7. 2018
Nakladatel
Elsevier
ISSN
2211-9264
Periodikum
Algal Research
Ročník
34
Číslo
1
Stát
Nizozemsko
Strany od
116
Strany do
125
Strany počet
10
URL
https://www.sciencedirect.com/science/article/pii/S2211926418302923
Plný text v Digitální knihovně
http://hdl.handle.net/11012/194778
BibTex
@article{BUT150270, author="Clemens {Troschl} and Katharina {Meixner} and Ines {Fritz} and Klaus {Leitner} and Patricia Alejandra {Palacios Romero} and Adriána {Kovalčík} and Petr {Sedláček} and Bernhard {Drosg}", title="Pilot-scale production of poly-β-hydroxybutyrate with the cyanobacterium Synechocytis sp. CCALA192 in a non-sterile tubular photobioreactor", journal="Algal Research", year="2018", volume="34", number="1", pages="116--125", doi="10.1016/j.algal.2018.07.011", issn="2211-9264", url="https://www.sciencedirect.com/science/article/pii/S2211926418302923" }