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MUSIL, M. KONEGGER, H. HON, J. BEDNÁŘ, D. DAMBORSKÝ, J.
Originální název
Computational Design of Stable and Soluble Biocatalysts
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
Natural enzymes are delicate biomolecules possessing only marginal thermodynamic stability. Poorly stable, misfolded, and aggregated proteins lead to huge economic losses in the biotechnology and biopharmaceutical industries. Consequently, there is a need to design optimized protein sequences that maximize stability, solubility, and activity over a wide range of temperatures and pH values, in buffers of different composition, and in the presence of organic co-solvents. This has created great interest in using computational methods to enhance biocatalysts robustness and solubility. Suitable methods include (i) energy calculations, (ii) machine learning, (iii) phylogenetic analyses and (iv) combinations of these approaches. We have witnessed impressive progress in the design of stable enzymes over the last two decades, but predictions of protein solubility and expressibility are scarce. Stabilizing mutations can be predicted accurately using available force fields, the number of sequences available for phylogenetic analyses is growing, and complex computational workflows are being implemented in intuitive web tools, enhancing the quality of protein stability predictions. Conversely, solubility predictors are limited by the lack of robust and balanced experimental data, an inadequate understanding of fundamental principles of protein aggregation, and a dearth of structural information on folding intermediates. Here we summarize recent progress in the development of computational tools for predicting protein stability and solubility, critically assess their strengths and weaknesses, and identify apparent gaps in data and knowledge. We also present perspectives on the computational design of stable and soluble biocatalysts.
Klíčová slova
Aggregation,Computational Design,Force Field,Expressibility,Machine Learning,Phylogenetic Analysis,Enzyme Stability,Enzyme Solubility
Autoři
MUSIL, M.; KONEGGER, H.; HON, J.; BEDNÁŘ, D.; DAMBORSKÝ, J.
Vydáno
1. 2. 2019
ISSN
2155-5435
Periodikum
ACS Catalysis
Ročník
9
Číslo
2
Stát
Spojené státy americké
Strany od
1033
Strany do
1054
Strany počet
22
URL
https://pubs.acs.org/doi/10.1021/acscatal.8b03613
BibTex
@article{BUT155120, author="Miloš {Musil} and Hannes {Konegger} and Jiří {Hon} and David {Bednář} and Jiří {Damborský}", title="Computational Design of Stable and Soluble Biocatalysts", journal="ACS Catalysis", year="2019", volume="9", number="2", pages="1033--1054", doi="10.1021/acscatal.8b03613", issn="2155-5435", url="https://pubs.acs.org/doi/10.1021/acscatal.8b03613" }