Detail publikace

The methodology for endoluminal irreversible electroporation in porcine models

GROLICH, T. ANDRAŠÍNA, T. CRHA, M. STEHLÍK, L. ROHAN, T. ČERVINKA, D. NOVOTNÁ, V. BERNARD, V. SVOBODOVÁ, I. VÁLEK, V.

Originální název

The methodology for endoluminal irreversible electroporation in porcine models

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The aim of this study was to describe the methodology of the surgical technique for endoluminal irreversible electroporation in the biliary tract performed within the perihilar region in porcine models. Endoluminal irreversible electroporation of the common bile duct was performed on eight porcine models using an endoluminal device inserted during laparotomy. The endoluminal device consisted of three electrodes 1 cm in length, attached at 120 degrees around the balloon catheter. The procedure was conducted with the following parameters: number of pulses 90, voltage of 1500 V between each couple of electrodes. Cross sectional imaging and histopathological assessment were employed for evaluations of the ablation zone. Models were sacrificed 24 h and 96 h after ablation. The treatment was successful in all porcine models. All animals survived the defined study period. Peri-ablation oedema within the hepatoduodenal ligament and adjacent liver tissue could be measured on post-procedural MRI or CT. Perforation in the site of ablation developed in one model. Histopathological examination showed heavy regressive changes of the ablated tissue. The elastic membranes of the adjacent portal vein were preserved in all models. In our experience, this novel endoluminal modality used within the perihilar region in porcine models is a feasible and well predictable procedure. Further studies should explore the optimal protocol of catheter-based ablation to limit complications.

Klíčová slova

Biliary ablation, magnetic resonance imaging, intraductal ablation, experimental study

Autoři

GROLICH, T.; ANDRAŠÍNA, T.; CRHA, M.; STEHLÍK, L.; ROHAN, T.; ČERVINKA, D.; NOVOTNÁ, V.; BERNARD, V.; SVOBODOVÁ, I.; VÁLEK, V.

Vydáno

23. 4. 2019

Nakladatel

Acta Veterinaria Brno

Místo

Brno

ISSN

1801-7576

Periodikum

ACTA VETERINARIA BRNO

Ročník

88

Číslo

2

Stát

Česká republika

Strany od

201

Strany do

205

Strany počet

5

URL

https://actavet.vfu.cz/88/2/0201/

Plný text v Digitální knihovně

http://hdl.handle.net/11012/184647

BibTex

@article{BUT157795,
  author="Tomáš {Grolich} and Tomáš {Andrašína} and Michal {Crha} and Ladislav {Stehlík} and Tomáš {Rohan} and Dalibor {Červinka} and Veronika {Novotná} and Vladan {Bernard} and Iva {Svobodová} and Vlastimil {Válek}",
  title="The methodology for endoluminal irreversible electroporation in porcine models",
  journal="ACTA VETERINARIA BRNO",
  year="2019",
  volume="88",
  number="2",
  pages="201--205",
  doi="10.2754/avb201988020201",
  issn="1801-7576",
  url="https://actavet.vfu.cz/88/2/0201/"
}