Detail publikace

Method for Analysis of Excitation-Emission Spectral Scans to Characterize Fluorescent Probes in Confocal Microscopy

ČMIEL, V. SKOPALÍK, J. BAIAZITOVA, L. PROVAZNÍK, I.

Originální název

Method for Analysis of Excitation-Emission Spectral Scans to Characterize Fluorescent Probes in Confocal Microscopy

Typ

článek v časopise - ostatní, Jost

Jazyk

angličtina

Originální abstrakt

The main aim of the study was to use the spectral excitation-emission 2D scanning followed by 2D spectral analysis in order to obtain an appropriate representation of the properties of the used dye in the original image of a stained cell. We used Di-4-ANNEPS voltage sensitive dye (Invitrogen) binding to an adult rat cardiomyocyte membrane to determine the stability of the dye and to characterize spatial differences of its spectral properties.

Klíčová slova

confocal microscopy; fluorescence; spectral scan; Stokes shift

Autoři

ČMIEL, V.; SKOPALÍK, J.; BAIAZITOVA, L.; PROVAZNÍK, I.

Vydáno

5. 8. 2019

Nakladatel

Cambridge University Press

Místo

New York

ISSN

1435-8115

Periodikum

Microscopy and Microanalysis

Ročník

25

Číslo

Suppl 2

Stát

Spojené státy americké

Strany od

1264

Strany do

1265

Strany počet

2

URL

https://www.cambridge.org/core/services/aop-cambridge-core/content/view/9BF44630933F77D35CE7544BF48EA85D/S1431927619007050a.pdf/method_for_analysis_of_excitationemission_spectral_scans_to_characterize_fluorescent_probes_in_confocal_microscopy.pdf

BibTex

@article{BUT157958,
  author="Vratislav {Čmiel} and Josef {Skopalík} and Larisa {Chmelíková} and Valentine {Provazník}",
  title="Method for Analysis of Excitation-Emission Spectral Scans to Characterize Fluorescent Probes in Confocal Microscopy",
  journal="Microscopy and Microanalysis",
  year="2019",
  volume="25",
  number="Suppl 2",
  pages="1264--1265",
  doi="10.1017/S1431927619007050",
  issn="1435-8115",
  url="https://www.cambridge.org/core/services/aop-cambridge-core/content/view/9BF44630933F77D35CE7544BF48EA85D/S1431927619007050a.pdf/method_for_analysis_of_excitationemission_spectral_scans_to_characterize_fluorescent_probes_in_confocal_microscopy.pdf"
}