Optimizing the Laser Ablation of Soft Tissues

konferenční sborník (ne článek)

angličtina

The issue of laser-matter interaction is of great importance in the material analysis using laser-ablation-based techniques, such as Laser-Induced Breakdown Spectroscopy (LIBS). The distribution of elements in soft tissues is an important source of information, which can facilitate diagnosing. LIBS is shown to be a viable option for qualitative imaging of specific elements in biological tissue material. The laser ablation is driven namely by the proper selection of laser wavelength and irradiance. Optimal soft tissue measurement parameters were determined by the pulse wavelength optimization process. Tested samples were mouse kidneys fixed in paraffin. Gradually, all possible wavelengths of laser pulse were tested: 1064 nm, 532 nm and 266 nm. The energy within the range from 1 mJ to 25 mJ, setting with a step of 5 mJ, was tested for energy laser pulse. The signal dependence of individual elements was monitored with respect to their concentration. Moreover, the resulting wavelength and laser energy values were determined based on the signal-to-noise and signal-to-background ratio calculation. The essence of this research is a fundamental study for future research on the distribution of the selected elements in the tumours. That could lead to the robustness increase in determining the type and extent of malignant lesions based on the deeper knowledge of tissue metabolism.

LIBS, soft tissues

Kristina Virostkova, Pavel Pořízka, Lucie Vrlíková, Marcela Buchtová, Jozef Kaiser

17. 9. 2019

Spektroskopická společnost Jana Marka Marci

Ke Karlovu 2027/3, 120 00 Praha 2 - Nové Město

978-80-88195-13-9

293

http://libs.ceitec.cz/files/281/213.pdf