Detail publikace

Tuning the surface coating of IONs toward efficient sonochemical tethering and sustained liberation of topoisomerase II poisons

MICHÁLKOVÁ, H. STRMISKA, V. KUDR, J. ŠKUBALOVÁ, Z. TESAŘOVÁ, B. ŠVEC, P. RICHTERA, L. ZÍTKA, O. ADAM, V. HEGER, Z.

Originální název

Tuning the surface coating of IONs toward efficient sonochemical tethering and sustained liberation of topoisomerase II poisons

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Background: Iron oxide nanoparticles (IONs) have been increasingly utilized in a wide spectrum of biomedical applications. Surface coatings of IONs can bestow a number of exceptional properties, including enhanced stability of IONs, increased loading of drugs or their controlled release. Methods: Using two-step sonochemical protocol, IONs were surface-coated with polyoxyethylene stearate, polyvinylpyrrolidone or chitosan for a loading of two distinct topo II poisons (doxorubicin and ellipticine). The cytotoxic behavior was tested in vitro against breast cancer (MDA-MB-231) and healthy epithelial cells (HEK-293 and HBL-100). In addition, biocompatibility studies (hemotoxicity, protein corona formation, binding of third complement component) were performed. Results: Notably, despite surface-coated IONs exhibited only negligible cytotoxicity, upon tethering with topo II poisons, synergistic or additional enhancement of cytotoxicity was found in MDA-MB-231 cells. Pronounced anti-migratory activity, DNA fragmentation, decrease in expression of procaspase-3 and enhancement of p53 expression were further identified upon exposure to surface-coated IONs with tethered doxorubicin and ellipticine. Moreover, surface-coated IONs nanoformulations of topo II poisons exhibited exceptional stability in human plasma with no protein corona and complement 3 binding, and only a mild induction of hemolysis in human red blood cells. Conclusion: The results imply a high potential of an efficient ultrasound-mediated surface functionalization of IONs as delivery vehicles to improve therapeutic efficiency of topo II poisons.

Klíčová slova

doxorubicin; ellipticine; iron oxide; nanoparticles; release kinetics

Autoři

MICHÁLKOVÁ, H.; STRMISKA, V.; KUDR, J.; ŠKUBALOVÁ, Z.; TESAŘOVÁ, B.; ŠVEC, P.; RICHTERA, L.; ZÍTKA, O.; ADAM, V.; HEGER, Z.

Vydáno

17. 9. 2019

Nakladatel

Dove Medical Press

ISSN

1178-2013

Periodikum

International Journal of Nanomedicine

Ročník

14

Číslo

1

Stát

Nový Zéland

Strany od

7609

Strany do

7624

Strany počet

16

URL

https://www.dovepress.com/tuning-the-surface-coating-of-ions-toward-efficient-sonochemical-tethe-peer-reviewed-article-IJN

Plný text v Digitální knihovně

http://hdl.handle.net/11012/181092

BibTex

@article{BUT160088,
  author="Hana {Michálková} and Vladislav {Strmiska} and Jiří {Kudr} and Zuzana {Škubalová} and Barbora {Tesařová} and Pavel {Švec} and Lukáš {Richtera} and Ondřej {Zítka} and Vojtěch {Adam} and Zbyněk {Heger}",
  title="Tuning the surface coating of IONs toward efficient sonochemical tethering and sustained liberation of topoisomerase II poisons",
  journal="International Journal of Nanomedicine",
  year="2019",
  volume="14",
  number="1",
  pages="7609--7624",
  doi="10.2147/IJN.S208810",
  issn="1178-2013",
  url="https://www.dovepress.com/tuning-the-surface-coating-of-ions-toward-efficient-sonochemical-tethe-peer-reviewed-article-IJN"
}