Detail publikace

Introducing the Newly Isolated BacteriumAneurinibacillussp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers-1. Isolation and Characterization of the Bacterium

PERNICOVÁ, I. NOVÁČKOVÁ, I. SEDLÁČEK, P. KOUŘILOVÁ, X. KALINA, M. KOVALČÍK, A. KOLLER, M. NEBESAROVA, J. KRZYZANEK, V. HRUBANOVA, K. MÁSILKO, J. SLANINOVÁ, E. OBRUČA, S.

Originální název

Introducing the Newly Isolated BacteriumAneurinibacillussp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers-1. Isolation and Characterization of the Bacterium

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Extremophilic microorganisms are considered being very promising candidates for biotechnological production of various products including polyhydroxyalkanoates (PHA). The aim of this work was to evaluate the PHA production potential of a novel PHA-producing thermophilic Gram-positive isolateAneurinibacillussp. H1. This organism was capable of efficient conversion of glycerol into poly(3-hydroxybutyrate) (P3HB), the homopolyester of 3-hydroxybutyrate (3HB). In flasks experiment, under optimal cultivation temperature of 45 degrees C, the P3HB content in biomass and P3HB titers reached 55.31% of cell dry mass and 2.03 g/L, respectively. Further, the isolate was capable of biosynthesis of PHA copolymers and terpolymers containing high molar fractions of 3-hydroxyvalerate (3HV) and 4-hydroxybutyrate (4HB). Especially 4HB contents in PHA were very high (up to 91 mol %) when 1,4-butanediol was used as a substrate. Based on these results, it can be stated thatAneurinibacillussp. H1 is a very promising candidate for production of PHA with tailored material properties.

Klíčová slova

polyhydroxyalkanoates; thermophiles; Aneurinibacillussp; P(3HB-co-4HB); P(3HB-co-3HV-co-4HB)

Autoři

PERNICOVÁ, I.; NOVÁČKOVÁ, I.; SEDLÁČEK, P.; KOUŘILOVÁ, X.; KALINA, M.; KOVALČÍK, A.; KOLLER, M.; NEBESAROVA, J.; KRZYZANEK, V.; HRUBANOVA, K.; MÁSILKO, J.; SLANINOVÁ, E.; OBRUČA, S.

Vydáno

29. 5. 2020

Nakladatel

MDPI

Místo

Switzerland

ISSN

2073-4360

Periodikum

Polymers

Ročník

12

Číslo

6

Stát

Švýcarská konfederace

Strany od

1

Strany do

13

Strany počet

13

URL

https://www.mdpi.com/2073-4360/12/6/1235

Plný text v Digitální knihovně

http://hdl.handle.net/11012/193675

BibTex

@article{BUT164652,
  author="Iva {Buchtíková} and Ivana {Nováčková} and Petr {Sedláček} and Xenie {Kouřilová} and Michal {Kalina} and Adriána {Kovalčík} and Martin {Koller} and Jana {Nebesářová} and Vladislav {Krzyžánek} and Kamila {Hrubanová} and Jiří {Másilko} and Eva {Slaninová} and Stanislav {Obruča}",
  title="Introducing the Newly Isolated BacteriumAneurinibacillussp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers-1. Isolation and Characterization of the Bacterium",
  journal="Polymers",
  year="2020",
  volume="12",
  number="6",
  pages="1--13",
  doi="10.3390/polym12061235",
  issn="2073-4360",
  url="https://www.mdpi.com/2073-4360/12/6/1235"
}