Detail publikace

Phenotypic and genomic analysis of isopropanol and 1,3-propanediol producer Clostridium diolis DSM 15410

SEDLÁŘ, K. VASYLKIVSKA, M. MUSILOVÁ, J. BRANSKÁ, B. PROVAZNÍK, I. PATÁKOVÁ, P.

Originální název

Phenotypic and genomic analysis of isopropanol and 1,3-propanediol producer Clostridium diolis DSM 15410

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Clostridium diolis DSM 15410 is a type strain of solventogenic clostridium capable of conducting isopropanol-butanol-ethanol fermentation. By studying its growth on different carbohydrates, we verified its ability to utilize glycerol and produce 1,3-propanediol and discovered its ability to produced isopropanol. Complete genome sequencing showed that its genome is a single circular chromosome and belongs to the cluster I (sensu scricto) of the genus Clostridium. By cultivation analysis we highlighted its specific behavior in comparison to two selected closely related strains. Despite the fact that several CRISPR loci were found, 16 putative prophages showed the ability to receive foreign DNA. Thus, the strain has the necessary features for future engineering of its 1,3-propanediol biosynthetic pathway and for the possible industrial utilization in the production of biofuels.

Klíčová slova

Clostridium diolis; complete genome; solventogenic; IBE; 1,3-propanediol

Autoři

SEDLÁŘ, K.; VASYLKIVSKA, M.; MUSILOVÁ, J.; BRANSKÁ, B.; PROVAZNÍK, I.; PATÁKOVÁ, P.

Vydáno

7. 11. 2020

Nakladatel

Elsevier

ISSN

0888-7543

Periodikum

GENOMICS

Ročník

113

Číslo

1

Stát

Spojené státy americké

Strany od

1109

Strany do

1119

Strany počet

11

URL

https://www.sciencedirect.com/science/article/pii/S088875432032005X

Plný text v Digitální knihovně

http://hdl.handle.net/11012/196477

BibTex

@article{BUT165852,
  author="Karel {Sedlář} and Maryna {Vasylkivska} and Jana {Musilová} and Barbora {Branská} and Valentine {Provazník} and Petra {Patáková}",
  title="Phenotypic and genomic analysis of isopropanol and 1,3-propanediol producer Clostridium diolis DSM 15410",
  journal="GENOMICS",
  year="2020",
  volume="113",
  number="1",
  pages="1109--1119",
  doi="10.1016/j.ygeno.2020.11.007",
  issn="0888-7543",
  url="https://www.sciencedirect.com/science/article/pii/S088875432032005X"
}