Detail publikace

Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies

NĚMČÁKOVÁ, I. BLAHOVÁ, L. RYŠÁNEK, P. BLANQUER, A. BAČÁKOVÁ, L. ZAJÍČKOVÁ, L.

Originální název

Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Amine-coated biodegradable materials based on synthetic polymers have a great potential for tissue remodeling and regeneration because of their excellent processability and bioactivity. In the present study, we have investigated the influence of various chemical compositions of amine plasma polymer (PP) coatings and the influence of the substrate morphology, represented by polystyrene culture dishes and polycaprolactone nanofibers (PCL NFs), on the behavior of vascular smooth muscle cells (VSMCs). Although all amine-PP coatings improved the initial adhesion of VSMCs, 7-day long cultivation revealed a clear preference for the coating containing about 15 at.% of nitrogen (CPA-33). The CPA-33 coating demonstrated the ideal combination of good water stability, a sufficient amine group content, and favorable surface wettability and morphology. The nanostructured morphology of amine-PP-coated PCL NFs successfully slowed the proliferation rate of VSMCs, which is essential in preventing restenosis of vascular replacements in vivo. At the same time, CPA-33-coated PCL NFs supported the continuous proliferation of VSMCs during 7-day long cultivation, with no significant increase in cytokine secretion by RAW 264.7 macrophages. The CPA-33 coating deposited on biodegradable PCL NFs therefore seems to be a promising material for manufacturing small-diameter vascular grafts, which are still lacking on the current market.

Klíčová slova

amine plasma polymer; bioactive coating; polycaprolactone nanofibers; substrate morphology; cell adhesion; cell proliferation

Autoři

NĚMČÁKOVÁ, I.; BLAHOVÁ, L.; RYŠÁNEK, P.; BLANQUER, A.; BAČÁKOVÁ, L.; ZAJÍČKOVÁ, L.

Vydáno

1. 12. 2020

Nakladatel

MDPI

Místo

BASEL

ISSN

1422-0067

Periodikum

International Journal of Molecular Sciences

Ročník

21

Číslo

24

Stát

Švýcarská konfederace

Strany od

1

Strany do

24

Strany počet

24

URL

https://www.mdpi.com/1422-0067/21/24/9467

Plný text v Digitální knihovně

http://hdl.handle.net/11012/196474

BibTex

@article{BUT169628,
  author="Ivana {Němčáková} and Lucie {Janů} and Petr {Ryšánek} and Andreu {Blanquer} and Lucie {Bačáková} and Lenka {Zajíčková}",
  title="Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies",
  journal="International Journal of Molecular Sciences",
  year="2020",
  volume="21",
  number="24",
  pages="1--24",
  doi="10.3390/ijms21249467",
  issn="1422-0067",
  url="https://www.mdpi.com/1422-0067/21/24/9467"
}