Potential functional capacity of a non-model organism Aneurinibacillus species H1

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angličtina

Polyhydroxyalkanoates (PHA) are polyesters similar to industrially processed synthetic polymers. Aneurinibacillus species H1 belongs to thermophilic gram-positive bacteria capable of accumulating the PHA in a high amount under certain conditions. Whereas the bacterium´s genetic information has not been studied for its ability to accumulate PHA yet, we bring the first genome assembly along with insight into its functional properties. The genome was assembled using sequencing data obtained from Oxford Nanopore MinION and Illumina MiSeq platforms. In the first step, the initial assembly from Nanopore reads was created. Subsequently, trimmed Illumina reads were mapped to the initial assembly with the result of final assembly. The final assembly was rearranged according to the replication origin (oriC) in a way that the DnaA gene is the first gene. To reveal potential functional capacity of the genome, we analysed particular protein coding genes and divide them according to clusters of orthologous genes (COG) using the eggNOG-mapper tool. Finally, we have predicted operons using the Operon-mapper tool. The presented insight into the genome is an ideal beginning for exploring the possibilities of polyhydroxyalkanoates production. The acquired knowledge will help with the future engineering of the strain Aneurinibacillus species H1. Moreover, the results can be generalized also for the accumulation of polyesters in related species. This work has been supported by grant project FCH/FEKT-J-21-7331. PhD study of Jana Musilova is supported by „Brno Ph.D. Talent Scholarship– Funded by the Brno City Municipality“.

Aneurinibacillus species H1; non-model organism

MUSILOVÁ, J.; KOUŘILOVÁ, X.; OBRUČA, S.; SEDLÁŘ, K.; LENGEROVÁ, M.; BEZDÍČEK, M.

18. 6. 2021