Detail publikace

Efficient Protein Transfection by Swarms of Chemically Powered Plasmonic Virus-Sized Nanorobots

RESSNEROVÁ, A. NOVOTNÝ, F. MICHÁLKOVÁ, H. PUMERA, M. ADAM, V. HEGER, Z.

Originální název

Efficient Protein Transfection by Swarms of Chemically Powered Plasmonic Virus-Sized Nanorobots

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Transfection is based on nonviral delivery of nucleic acids or proteins into cells. Viral approaches are being used; nevertheless, their translational capacity is nowadays decreasing due to persistent fear of their safety, therefore creating space for the field of nanotechnology. However, nanomedical approaches introducing static nanoparticles for the delivery of biologically active molecules are very likely to be overshadowed by the vast potential of nanorobotics. We hereby present a rapid nonviral transfection of protein into a difficult-to-transfect prostate cancer cell line facilitated by chemically powered rectangular virus-sized (68 nm x 33 nm) nanorobots. The enhanced diffusion of these biocompatible nanorobots is the key to their fast internalization into cells, happening in a matter of minutes and being up to 6-fold more efficient compared to static nanorobots in a nonfueled environment. The Au/Ag plasmonic nature of these nanorobots makes them simply traceable and allows for their detailed subcellular localization. Protein transfection mediated by such nanorobots is an important step forward, challenging the field of nanomedicine and having potential in future translational medical research.

Klíčová slova

transfection; nanorobots; metallothionein; protein delivery; protein transfection

Autoři

RESSNEROVÁ, A.; NOVOTNÝ, F.; MICHÁLKOVÁ, H.; PUMERA, M.; ADAM, V.; HEGER, Z.

Vydáno

24. 8. 2021

Nakladatel

American Chemical Society

ISSN

1936-0851

Periodikum

ACS Nano

Ročník

15

Číslo

8

Stát

Spojené státy americké

Strany od

12899

Strany do

12910

Strany počet

12

URL

https://pubs.acs.org/doi/10.1021/acsnano.1c01172

Plný text v Digitální knihovně

http://hdl.handle.net/11012/203120

BibTex

@article{BUT172541,
  author="Alžběta {Ressnerová} and Filip {Novotný} and Hana {Michálková} and Martin {Pumera} and Vojtěch {Adam} and Zbyněk {Heger}",
  title="Efficient Protein Transfection by Swarms of Chemically Powered Plasmonic Virus-Sized Nanorobots",
  journal="ACS Nano",
  year="2021",
  volume="15",
  number="8",
  pages="12899--12910",
  doi="10.1021/acsnano.1c01172",
  issn="1936-0851",
  url="https://pubs.acs.org/doi/10.1021/acsnano.1c01172"
}