Detail publikace

Full-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopy

TURČANOVÁ, M. HRTOŇ, M. DVOŘÁK, P. NOVÁK, K. HERMANOVÁ, M. BEDNAŘÍK, Z. POLZER, S. BURŠA, J.

Originální název

Full-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopy

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

A novel method for semiautomated assessment of directions of collagen fibers in soft tissues using histological image analysis is presented. It is based on multiple rotated images obtained via polarized light microscopy without any additional components, i.e., with just two polarizers being either perpendicular or nonperpendicular (rotated). This arrangement breaks the limitation of 90° periodicity of polarized light intensity and evaluates the in-plane fiber orientation over the whole 180° range accurately and quickly. After having verified the method, we used histological specimens of porcine Achilles tendon and aorta to validate the proposed algorithm and to lower the number of rotated images needed for evaluation. Our algorithm is capable to analyze 5·105 pixels in one micrograph in a few seconds and is thus a powerful and cheap tool promising a broad application in detection of collagen fiber distribution in soft tissues.

Klíčová slova

soft tissue; histological slice; collagen; fiber direction; polarized light microscopy; histogram

Autoři

TURČANOVÁ, M.; HRTOŇ, M.; DVOŘÁK, P.; NOVÁK, K.; HERMANOVÁ, M.; BEDNAŘÍK, Z.; POLZER, S.; BURŠA, J.

Vydáno

28. 11. 2021

Nakladatel

Hindawi

Místo

London, United Kingdom

ISSN

2314-6141

Periodikum

BioMed Research International

Ročník

2021

Číslo

1

Stát

Spojené státy americké

Strany od

1

Strany do

13

Strany počet

13

URL

https://www.hindawi.com/journals/bmri/2021/6879765/

Plný text v Digitální knihovně

http://hdl.handle.net/11012/203098

BibTex

@article{BUT175037,
  author="Michaela {Turčanová} and Martin {Hrtoň} and Petr {Viewegh} and Kamil {Novák} and Markéta {Hermanová} and Zdeněk {Bednařík} and Stanislav {Polzer} and Jiří {Burša}",
  title="Full-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopy",
  journal="BioMed Research International",
  year="2021",
  volume="2021",
  number="1",
  pages="1--13",
  doi="10.1155/2021/6879765",
  issn="2314-6141",
  url="https://www.hindawi.com/journals/bmri/2021/6879765/"
}