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MRÁZOVÁ, K. NOVÁČKOVÁ, I. KOUŘILOVÁ, X. OBRUČA, S. KRZYŽÁNEK, V.
Originální název
The first insight on changes in PHA granules after extraction from extremophilic microorganisms
Typ
abstrakt
Jazyk
angličtina
Originální abstrakt
Polyhydroxyalkanoates (PHA) are microbial polyesters that are accumulated in form of intracellular granules by many prokaryotic organisms. One of the interesting properties of PHA granules is their extreme elasticity. It was found out that PHA stay elastic even at temperatures of liquid nitrogen. In the experiment, cells frozen in liquid nitrogen were freeze-fractured and imaged in a scanning electron microscope with cryostage (cryoSEM). PHA could be then observed sticking out of the fractured cells. In our work, we focused on the extraction of PHA from halophilic Halomonas halophila and thermophilic Schlegelella thermodepolymerans and subsequently on the study of changes in their morphology and elastic properties after extraction using cryoSEM.
Klíčová slova
PHA, freeze-fracture, cryo SEM, Halomonas halophila, Schlegelella thermodepolymerans
Autoři
MRÁZOVÁ, K.; NOVÁČKOVÁ, I.; KOUŘILOVÁ, X.; OBRUČA, S.; KRZYŽÁNEK, V.
Vydáno
13. 9. 2021
Strany od
81
Strany do
Strany počet
1
BibTex
@misc{BUT175387, author="Kateřina {Mrázová} and Ivana {Nováčková} and Xenie {Kouřilová} and Stanislav {Obruča} and Vladislav {Krzyžánek}", title="The first insight on changes in PHA granules after extraction from extremophilic microorganisms", booktitle="Microscopy 2021: Conference", year="2021", series="1", edition="1", pages="81--81", note="abstract" }