Detail publikace

Modifications of Parylene by Microstructures and Selenium Nanoparticles: Evaluation of Bacterial and Mesenchymal Stem Cell Viability

PEKÁRKOVÁ, J. GABLECH, I. FIALOVÁ, T. BÍLEK, O. FOHLEROVÁ, Z.

Originální název

Modifications of Parylene by Microstructures and Selenium Nanoparticles: Evaluation of Bacterial and Mesenchymal Stem Cell Viability

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Parylene-based implants or coatings introduce surfaces suffering from bacteria colonization. Here, we synthesized polyvinylpyrrolidone-stabilized selenium nanoparticles (SeNPs) as the antibacterial agent, and various approaches are studied for their reproducible adsorption, and thus the modification of parylene-C-coated glass substrate. The nanoparticle deposition process is optimized in the nanoparticle concentration to obtain evenly distributed NPs on the flat parylene-C surface. Moreover, the array of parylene-C micropillars is fabricated by the plasma etching of parylene-C on a silicon wafer, and the surface is modified with SeNPs. All designed surfaces are tested against two bacterial pathogens, Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive). The results show no antibacterial effect toward S. aureus, while some bacteriostatic effect is observed for E. coli on the flat and microstructured parylene. However, SeNPs did not enhance the antibacterial effect against both bacteria. Additionally, all designed surfaces show cytotoxic effects toward mesenchymal stem cells at high SeNP deposition. These results provide valuable information about the potential antibacterial treatment of widely used parylene-C in biomedicine.

Klíčová slova

parylene-C; micropillars; selenium nanoparticles; biocompatibility; antimicrobial

Autoři

PEKÁRKOVÁ, J.; GABLECH, I.; FIALOVÁ, T.; BÍLEK, O.; FOHLEROVÁ, Z.

Vydáno

3. 12. 2021

Nakladatel

FRONTIERS MEDIA SA

Místo

LAUSANNE

ISSN

2296-4185

Periodikum

Frontiers in Bioengineering and Biotechnology

Ročník

9

Číslo

1

Stát

Švýcarská konfederace

Strany od

1

Strany do

11

Strany počet

11

URL

https://www.frontiersin.org/articles/10.3389/fbioe.2021.782799/full

Plný text v Digitální knihovně

http://hdl.handle.net/11012/203324

BibTex

@article{BUT175415,
  author="Jana {Pekárková} and Imrich {Gablech} and Tatiana {Fialová} and Ondřej {Bílek} and Zdenka {Fohlerová}",
  title="Modifications of Parylene by Microstructures and Selenium Nanoparticles: Evaluation of Bacterial and Mesenchymal Stem Cell Viability",
  journal="Frontiers in Bioengineering and Biotechnology",
  year="2021",
  volume="9",
  number="1",
  pages="1--11",
  doi="10.3389/fbioe.2021.782799",
  issn="2296-4185",
  url="https://www.frontiersin.org/articles/10.3389/fbioe.2021.782799/full"
}