Detail publikace

Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation

ŠAFAŘÍKOVÁ, E. EHLICH, J. STŘÍTESKÝ, S. VALA, M. WEITER, M. PACHERNÍK, J. KUBALA, L. VÍTEČEK, J.

Originální název

Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Organic semiconductors are constantly gaining interest in regenerative medicine. Their tunable physico-chemical properties, including electrical conductivity, are very promising for the control of stem-cell differentiation. However, their use for combined material-based and electrical stimulation remains largely underexplored. Therefore, we carried out a study on whether a platform based on the conductive polymer poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) can be beneficial to the differentiation of mouse embryonic stem cells (mESCs). The platform was prepared using the layout of a standard 24-well cell-culture plate. Polyethylene naphthalate foil served as the substrate for the preparation of interdigitated gold electrodes by physical vapor deposition. The PEDOT:PSS pattern was fabricated by precise screen printing over the gold electrodes. The PEDOT:PSS platform was able to produce higher electrical current with the pulsed-direct-current (DC) electrostimulation mode (1 Hz, 200 mV/mm, 100 ms pulse duration) compared to plain gold electrodes. There was a dominant capacitive component. In proof-of-concept experiments, mESCs were able to respond to such electrostimulation by membrane depolarization and elevation of cytosolic calcium. Further, the PEDOT:PSS platform was able to upregulate cardiomyogenesis and potentially inhibit early neurogenesis per se with minor contribution of electrostimulation. Hence, the present work highlights the large potential of PEDOT:PSS in regenerative medicine.

Klíčová slova

conductive polymer; PEDOT; PSS; screen print; embryonic stem cells; electrostimulation

Autoři

ŠAFAŘÍKOVÁ, E.; EHLICH, J.; STŘÍTESKÝ, S.; VALA, M.; WEITER, M.; PACHERNÍK, J.; KUBALA, L.; VÍTEČEK, J.

Vydáno

20. 1. 2022

Nakladatel

MDPI

Místo

BASEL

ISSN

1422-0067

Periodikum

International Journal of Molecular Sciences

Ročník

23

Číslo

3

Stát

Švýcarská konfederace

Strany od

1

Strany do

16

Strany počet

16

URL

https://www.mdpi.com/1422-0067/23/3/1107

Plný text v Digitální knihovně

http://hdl.handle.net/11012/204601

BibTex

@article{BUT177314,
  author="Eva {Šafaříková} and Jiří {Ehlich} and Stanislav {Stříteský} and Martin {Vala} and Martin {Weiter} and Jiří {Pacherník} and Lukáš {Kubala} and Jan {Víteček}",
  title="Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation",
  journal="International Journal of Molecular Sciences",
  year="2022",
  volume="23",
  number="3",
  pages="1--16",
  doi="10.3390/ijms23031107",
  issn="1422-0067",
  url="https://www.mdpi.com/1422-0067/23/3/1107"
}