Detail publikace

Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov.

SEDLÁČEK, I. HOLOCHOVÁ, P. BUSSE, H. KOUBLOVÁ, V. KRÁLOVÁ, S. ŠVEC, P. SOBOTKA, R. STAŇKOVÁ, E. PILNÝ, J. ŠEDO, O. SMOLÍKOVÁ, J. SEDLÁŘ, K.

Originální název

Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov.

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

A group of seven bacterial strains producing blue-purple pigmented colonies on R2A agar was isolated from freshwater samples collected in a deglaciated part of James Ross Island and Eagle Island, Antarctica, from 2017-2019. The isolates were psychrophilic, oligotrophic, resistant to chloramphenicol, and exhibited strong hydrolytic activities. To clarify the taxonomic position of these isolates, a polyphasic taxonomic approach was applied based on sequencing of the 16S rRNA, gyrB and lepA genes, whole-genome sequencing, rep-PCR, MALDI-TOF MS, chemotaxonomy analyses and biotyping. Phylogenetic analysis of the 16S rRNA gene sequences revealed that the entire group are representatives of the genus Massilia. The closest relatives of the reference strain P8398(T) were Massilia atriviolacea, Massilia violaceinigra, Massilia rubra, Massilia mucilaginosa, Massilia aquatica, Massilia frigida, Massilia glaciei and Massilia eurypsychrophila with a pairwise similarity of 98.6-100% in the 16S rRNA. The subsequent gyrB and lepA sequencing results showed the novelty of the analysed group, and the average nucleotide identity and digital DNA-DNA hybridisation values clearly proved that P8398(T) represents a distinct Massilia species. After all these results, we nominate a new species with the proposed name Massilia antarctica sp. nov. The type strain is P8398(T) (= CCM 8941(T) = LMG 32108(T)).

Klíčová slova

Massilia; violacein; psychrophilic; description; whole-genome sequencing; Antarctica

Autoři

SEDLÁČEK, I.; HOLOCHOVÁ, P.; BUSSE, H.; KOUBLOVÁ, V.; KRÁLOVÁ, S.; ŠVEC, P.; SOBOTKA, R.; STAŇKOVÁ, E.; PILNÝ, J.; ŠEDO, O.; SMOLÍKOVÁ, J.; SEDLÁŘ, K.

Vydáno

24. 3. 2022

Nakladatel

MDPI

Místo

BASEL

ISSN

2076-2607

Periodikum

Microorganisms

Ročník

10

Číslo

4

Stát

Švýcarská konfederace

Strany od

1

Strany do

14

Strany počet

14

URL

https://www.mdpi.com/2076-2607/10/4/704/htm

Plný text v Digitální knihovně

http://hdl.handle.net/11012/204462

BibTex

@article{BUT177721,
  author="Ivo {Sedláček} and Pavla {Holochová} and Hans-Jürgen {Busse} and Vendula {Koublová} and Stanislava {Králová} and Pavel {Švec} and Roman {Sobotka} and Eva {Staňková} and Jan {Pilný} and Ondrej {Šedo} and Jana {Smolíková} and Karel {Sedlář}",
  title="Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov.",
  journal="Microorganisms",
  year="2022",
  volume="10",
  number="4",
  pages="1--14",
  doi="10.3390/microorganisms10040704",
  issn="2076-2607",
  url="https://www.mdpi.com/2076-2607/10/4/704/htm"
}