Detail publikace

Hyaluronan interactions with cationic surfactants-Insights from fluorescence resonance energy transfer and anisotropy techniques

HOLÍNKOVÁ, P. MRAVEC, F. VENEROVÁ, T. CHANG, C. PEKAŘ, M.

Originální název

Hyaluronan interactions with cationic surfactants-Insights from fluorescence resonance energy transfer and anisotropy techniques

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Interactions of hyaluronan with micelles formed by cationic surfactants were studied by the time-resolved measurement of fluorescence resonance energy transfer (FRET) using perylene and fluorescein as probes. Two surfactants were studied - Cetyltrimethylammonium bromide (CTAB) and Septonex. In pure micellar solutions, the same values of FRET efficiency were found for both surfactants, but values for the binding of the probe pair were lower for Septonex micelles than in the case of CTAB. This was attributed to steric effects of the carbethoxy group in the Septonex polar head. Upon the addition of hyaluronan, decreased FRET efficiency and increased binding were detected in comparison with pure surfactants. To resolve the structure of the formed aggregates, steady state and time-resolved fluorescence anisotropy was employed as an additional technique. All results indicated that cationic micelles bind to hyaluronan forming a pearl necklace structure with micelles of smaller size compared to pure surfactant. Besides theoretical interest, the studied polyelectrolyte-surfactant system may be interesting for the formulation of drug delivery systems.

Klíčová slova

Fluorescence anisotropy; Fo ?rster resonance energy transfer; Hyaluronan; Surfactants

Autoři

HOLÍNKOVÁ, P.; MRAVEC, F.; VENEROVÁ, T.; CHANG, C.; PEKAŘ, M.

Vydáno

30. 6. 2022

Nakladatel

ELSEVIER

Místo

AMSTERDAM

ISSN

0141-8130

Periodikum

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Ročník

211

Číslo

1

Stát

Nizozemsko

Strany od

107

Strany do

115

Strany počet

9

URL

https://www.sciencedirect.com/science/article/abs/pii/S0141813022010376?via%3Dihub

BibTex

@article{BUT178267,
  author="Petra {Holínková} and Filip {Mravec} and Tereza {Venerová} and Chien-Hsiang {Chang} and Miloslav {Pekař}",
  title="Hyaluronan interactions with cationic surfactants-Insights from fluorescence resonance energy transfer and anisotropy techniques",
  journal="INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES",
  year="2022",
  volume="211",
  number="1",
  pages="107--115",
  doi="10.1016/j.ijbiomac.2022.05.067",
  issn="0141-8130",
  url="https://www.sciencedirect.com/science/article/abs/pii/S0141813022010376?via%3Dihub"
}