Detail publikace

Coherence-encoded synthetic aperture for super-resolution quantitative phase imaging

ĎURIŠ, M. BOUCHAL, P. ROVENSKÁ, K. CHMELÍK, R.

Originální název

Coherence-encoded synthetic aperture for super-resolution quantitative phase imaging

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Quantitative phase imaging (QPI) has quickly established its role in identifying rare events and screening in biomedicine or automated image data analysis using artificial intelligence. These and many other applications share the requirement for extensive high-quality datasets, which is challenging to meet because the invariance of the space-bandwidth product (SBP) fundamentally limits the microscope system throughput. Here, we present a method to overcome the SBP limit by achieving QPI super-resolution using a synthetic aperture approach in a holographic microscope with a partially coherent broad source illumination. We exploit intrinsic coherence-gating properties of the partially coherent light combined with the oblique illumination provided by the diffraction on a simple phase grating placed in proximity of the specimen. We sequentially coherence gate the light scattered into each grating's diffraction order, and we use the acquired images to synthesize QPI with significantly increased spatial frequency bandwidth. The resolution of QPI is increased substantially beyond Abbe's diffraction limit while a large field of view of low numerical aperture objectives is kept. This paper presents a thorough theoretical treatment of the coherence-gated imaging process supplemented by a detailed measurement methodology. The capability of the proposed method is demonstrated by imaging a phase resolution target and biological specimens. We envision our work providing an easily implementable super-resolution QPI method particularly suitable for high-throughput biomedical applications.

Klíčová slova

RESOLUTION IMPROVEMENT; HOLOGRAPHIC MICROSCOPY; DIGITAL HOLOGRAPHY;Super-resolution

Autoři

ĎURIŠ, M.; BOUCHAL, P.; ROVENSKÁ, K.; CHMELÍK, R.

Vydáno

1. 4. 2022

Nakladatel

AIP Publishing

ISSN

2378-0967

Periodikum

APL Photonics

Ročník

7

Číslo

4

Stát

Spojené státy americké

Strany od

1

Strany do

10

Strany počet

10

URL

https://aip.scitation.org/doi/10.1063/5.0081134

Plný text v Digitální knihovně

http://hdl.handle.net/11012/209179

BibTex

@article{BUT178555,
  author="Miroslav {Ďuriš} and Petr {Bouchal} and Katarína {Rovenská} and Radim {Chmelík}",
  title="Coherence-encoded synthetic aperture for super-resolution quantitative phase imaging",
  journal="APL Photonics",
  year="2022",
  volume="7",
  number="4",
  pages="1--10",
  doi="10.1063/5.0081134",
  issn="2378-0967",
  url="https://aip.scitation.org/doi/10.1063/5.0081134"
}