Detail publikace

Effect of Polymeric Nanoparticles with Entrapped Fish Oil or Mupirocin on Skin Wound Healing Using a Porcine Model

KOMPRDA, T. SLÁDEK, Z. VÍCENOVÁ, M. SIMONOVÁ, J. FRANKE, G. LIPOVÝ, B. MATEJOVIČOVÁ, M. KACVINSKÁ, K. SABLIOV, C. ASTETE, C. LEVÁ, L. POPELKOVÁ, V. BÁTIK, A. VOJTOVÁ, L.

Originální název

Effect of Polymeric Nanoparticles with Entrapped Fish Oil or Mupirocin on Skin Wound Healing Using a Porcine Model

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The utilization of poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs) with entrapped fish oil (FO) loaded in collagen-based scaffolds for cutaneous wound healing using a porcine model is unique for the present study. Full-depth cutaneous excisions (5 x 5 cm) on the pig dorsa were treated with pure collagen scaffold (control, C), empty PLGA NPs (NP), FO, mupirocin (MUP), PLGA NPs with entrapped FO (NP/FO) and PLGA NPs with entrapped MUP (NP/MUP). The following markers were evaluated on days 0, 3, 7, 14 and 21 post-excision: collagen, hydroxyproline (HP), angiogenesis and expressions of the COX2, EGF, COL1A1, COL1A3, TGFB1, VEGFA, CCL5 and CCR5 genes. The hypothesis that NP/FO treatment is superior to FO alone and that it is comparable to NP/MUP was tested. NP/FO treatment increased HP in comparison with both FO alone and NP/MUP (day 14) but decreased (p < 0.05) angiogenesis in comparison with FO alone (day 3). NP/FO increased (p < 0.05) the expression of the CCR5 gene (day 3) and tended (p > 0.05) to increase the expressions of the EGF (day 7, day 14), TGFB1 (day 21) and CCL5 (day 7, day 21) genes as compared with NP/MUP. NP/FO can be suggested as a suitable alternative to NP/MUP in cutaneous wound treatment.

Klíčová slova

cutaneous wounds; polyunsaturated fatty acids n-3; nanoparticles; poly(lactic-co-glycolic) acid; mupirocin; collagen; hydroxyproline; cyclooxygenase-2; transforming growth factor

Autoři

KOMPRDA, T.; SLÁDEK, Z.; VÍCENOVÁ, M.; SIMONOVÁ, J.; FRANKE, G.; LIPOVÝ, B.; MATEJOVIČOVÁ, M.; KACVINSKÁ, K.; SABLIOV, C.; ASTETE, C.; LEVÁ, L.; POPELKOVÁ, V.; BÁTIK, A.; VOJTOVÁ, L.

Vydáno

11. 7. 2022

Nakladatel

MDPI

Místo

BASEL

ISSN

1422-0067

Periodikum

International Journal of Molecular Sciences

Ročník

23

Číslo

14

Stát

Švýcarská konfederace

Strany od

7663

Strany do

7682

Strany počet

20

URL

https://www.mdpi.com/1422-0067/23/14/7663

BibTex

@article{BUT180332,
  author="Tomáš {Komprda} and Zbyšek {Sládek} and Monika {Vícenová} and Jana {Simonová} and Gabriela {Franke} and Břetislav {Lipový} and Milena {Matejovičová} and Katarína {Verčimáková} and Cristina {Sabliov} and Carlos E. {Astete} and Lenka {Levá} and Vendula {Popelková} and Andrej {Bátik} and Lucy {Vojtová}",
  title="Effect of Polymeric Nanoparticles with Entrapped Fish Oil or Mupirocin on Skin Wound Healing Using a Porcine Model",
  journal="International Journal of Molecular Sciences",
  year="2022",
  volume="23",
  number="14",
  pages="20",
  doi="10.3390/ijms23147663",
  issn="1422-0067",
  url="https://www.mdpi.com/1422-0067/23/14/7663"
}