Detail publikace

Demolded hollow high aspect-ratio parylene-C micropillars for real-time mechanosensing applications

CHMELÍKOVÁ, L. FECKO, P. CHMELÍK, J. SKÁCEL, J. OTÁHAL, A. FOHLEROVÁ, Z.

Originální název

Demolded hollow high aspect-ratio parylene-C micropillars for real-time mechanosensing applications

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Cells generate mechanical forces to maintain normal cellular function or play a role in developing pathological processes. The mechanical force called the traction force can be estimated from the pillar deflection using a polymeric micropillar array. Here, we develop a transparent membrane of hollow coneshape high aspect ratio (AR) parylene-C micropillars by the molding method. The membrane of the pillar array is exposed after the etching of the silicon mold and the residual silicon serves as the cultivation chamber. The AR and spring constant (k) of micropillars are estimated to AR ≈ 10 and k ≈ 0.349 N·m−1. The spring constant of developing micropillars is 3.5-times decreased compared to cylindrical, non-hollow pillars. This slightly tune the elastic properties of micropillars. The array is further shown as the mechanosensor detecting the change of cellular tension during hyperosmotic stress. The traction force of cancerous PC-3 cells is estimated from pillar deflections by image analysis. Additionally, the cell volume and surface area are measured using a digital holographic microscope (DHM). The results show that the molding technique can be used to develop high AR parylene-C micropillars and that this array can serve as the mechanosensor of cellular processes.

Klíčová slova

Micropillars, Parylene, Molding, Traction force, Chemical vapor deposition

Autoři

CHMELÍKOVÁ, L.; FECKO, P.; CHMELÍK, J.; SKÁCEL, J.; OTÁHAL, A.; FOHLEROVÁ, Z.

Vydáno

22. 2. 2023

Nakladatel

ELSEVIER

ISSN

2352-9407

Periodikum

Applied Materials Today

Ročník

31

Číslo

1

Stát

Nizozemsko

Strany od

1

Strany do

12

Strany počet

12

URL

https://www.webofscience.com/wos/woscc/full-record/WOS:000924683700001

BibTex

@article{BUT181441,
  author="Larisa {Chmelíková} and Peter {Fecko} and Jiří {Chmelík} and Josef {Skácel} and Alexandr {Otáhal} and Zdenka {Fohlerová}",
  title="Demolded hollow high aspect-ratio parylene-C micropillars for real-time mechanosensing applications",
  journal="Applied Materials Today",
  year="2023",
  volume="31",
  number="1",
  pages="1--12",
  doi="10.1016/j.apmt.2023.101736",
  issn="2352-9407",
  url="https://www.webofscience.com/wos/woscc/full-record/WOS:000924683700001"
}