Detail publikace

A mid-infrared lab-on-a-chip for dynamic reaction monitoring

HINKOV, B. PILAT, F. LUX, L. SOUZA, P. DAVID, M. SCHWAIGHOFER, A. RISTANIC, D. SCHWARZ, B. DETZ, H. ANDREWS, A. LENDL, B. STRASSER, G.

Originální název

A mid-infrared lab-on-a-chip for dynamic reaction monitoring

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Mid-infrared spectroscopy is a sensitive and selective technique for probing molecules in the gas or liquid phase. Investigating chemical reactions in bio-medical applications such as drug production is recently gaining particular interest. However, monitoring dynamic processes in liquids is commonly limited to bulky systems and thus requires time-consuming offline analytics. In this work, we show a next-generation, fully-integrated and robust chip-scale sensor for online measurements of molecule dynamics in a liquid solution. Our fingertip-sized device utilizes quantum cascade technology, combining the emitter, sensing section and detector on a single chip. This enables real-time measurements probing only microliter amounts of analyte in an in situ configuration. We demonstrate time-resolved device operation by analyzing temperature-induced conformational changes of the model protein bovine serum albumin in heavy water. Quantitative measurements reveal excellent performance characteristics in terms of sensor linearity, wide coverage of concentrations, extending from 0.075 mg ml(-1) to 92 mg ml(-1) and a 55-times higher absorbance than state-of-the-art bulky and offline reference systems. Rapid investigation of chemical reactions is a challenge in bio-medical analysis. Here, the authors demonstrate sensitive in-situ real-time reaction-monitoring of conformational changes in protein solution, based on a fingertip-sized mid-IR lab-on-a-chip.

Klíčová slova

BOVINE SERUM-ALBUMIN; QUANTUM-CASCADE-LASER; INFRARED FT-IR; DISTRIBUTED-FEEDBACK; SECONDARY STRUCTURE; SPECTROSCOPY; SENSORS; CONFORMATION; MODULATION; PROTEINS

Autoři

HINKOV, B.; PILAT, F.; LUX, L.; SOUZA, P.; DAVID, M.; SCHWAIGHOFER, A.; RISTANIC, D.; SCHWARZ, B.; DETZ, H.; ANDREWS, A.; LENDL, B.; STRASSER, G.

Vydáno

13. 8. 2022

Nakladatel

NATURE PORTFOLIO

Místo

BERLIN

ISSN

2041-1723

Periodikum

NATURE COMMUNICATIONS

Ročník

13

Číslo

1

Stát

Spojené království Velké Británie a Severního Irska

Strany počet

10

URL

https://www.nature.com/articles/s41467-022-32417-7

BibTex

@article{BUT181647,
  author="Borislav {Hinkov} and Florian {Pilat} and Laurin {Lux} and Patricia L. {Souza} and Mauro {David} and Andreas {Schwaighofer} and Daniela {Ristanic} and Benedikt {Schwarz} and Hermann {Detz} and Aaron M. {Andrews} and Bernhard {Lendl} and Gottfried {Strasser}",
  title="A mid-infrared lab-on-a-chip for dynamic reaction monitoring",
  journal="NATURE COMMUNICATIONS",
  year="2022",
  volume="13",
  number="1",
  pages="10",
  doi="10.1038/s41467-022-32417-7",
  issn="2041-1723",
  url="https://www.nature.com/articles/s41467-022-32417-7"
}