Detail publikace

Insight into peculiar adhesion of cells to plasma-chemically prepared multifunctional "amino-glue" surfaces

BUCHTELOVÁ, M. JANŮ, L. NEČAS, D. KŘÍŽKOVÁ, P. BARTOŠÍKOVÁ, J. MEDALOVÁ, J. KOLSKÁ, Z. HEGEMANN, D. ZAJÍČKOVÁ, L.

Originální název

Insight into peculiar adhesion of cells to plasma-chemically prepared multifunctional "amino-glue" surfaces

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Plasma polymers (PPs) can easily modify material surfaces to improve their bio-applicability due to match-made surface-free energy and functionality. However, cell adhesion to PPs typically composed of various functional groups has not yet been fully understood. We explain the origin of strong resistance to trypsin treatment previously noted for nonendothelial cells on amine PPs. It is caused mainly by nonspecific adhesion of negatively charged parts of transmembrane proteins to the positively charged amine PP surface, enabled by thin glycocalyx. However, endothelial cells are bound primarily by their thick, negatively charged glycocalyx and sporadically by integrins in kinetic traps, both cleaved by trypsin. Cell scratching by atomic force microscopy tip confirmed the correlation of trypsin resistance to the strength of cell adhesion.

Klíčová slova

amine plasma polymer; carboxyl plasma polymer; cell adhesion; glycocalyx; nonspecific adhesion

Autoři

BUCHTELOVÁ, M.; JANŮ, L.; NEČAS, D.; KŘÍŽKOVÁ, P.; BARTOŠÍKOVÁ, J.; MEDALOVÁ, J.; KOLSKÁ, Z.; HEGEMANN, D.; ZAJÍČKOVÁ, L.

Vydáno

1. 6. 2023

Nakladatel

Wiley-VCH

Místo

WEINHEIM

ISSN

1612-8869

Periodikum

Plasma Processes and Polymers

Ročník

20

Číslo

6

Stát

Spolková republika Německo

Strany od

1

Strany do

15

Strany počet

15

URL

https://onlinelibrary.wiley.com/doi/10.1002/ppap.202200157

Plný text v Digitální knihovně

http://hdl.handle.net/11012/213691

BibTex

@article{BUT183943,
  author="Martina {Janůšová} and Lucie {Janů} and David {Nečas} and Petra {Křížková} and Jana {Bartošíková} and Jiřina {Medalová} and Zdeňka {Kolská} and Dirk {Hegemann} and Lenka {Zajíčková}",
  title="Insight into peculiar adhesion of cells to plasma-chemically prepared multifunctional {"}amino-glue{"} surfaces",
  journal="Plasma Processes and Polymers",
  year="2023",
  volume="20",
  number="6",
  pages="15",
  doi="10.1002/ppap.202200157",
  issn="1612-8869",
  url="https://onlinelibrary.wiley.com/doi/10.1002/ppap.202200157"
}