Detail publikace

Inverted repeats in the monkeypox virus genome are hot spots for mutation

DOBROVOLNÁ, M. WARNER E. F. BIDULA S. BRÁZDA, V.

Originální název

Inverted repeats in the monkeypox virus genome are hot spots for mutation

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The current monkeypox virus (MPXV) strain differs from the strain arising in 2018 by 50+ single nucleotide polymorphisms (SNPs) and is mutating much faster than expected. The cytidine deaminase apolipoprotein B messenger RNA editing enzyme, catalytic subunit B (APOBEC3) was hypothesized to be driving this increased mutation. APOBEC has recently been identified to preferentially mutate cruciform DNA secondary structures formed by inverted repeats (IRs). IRs were recently identified as hot spots for mutation in severe acute respiratory syndrome coronavirus 2, and we aimed to identify whether IRs were also hot spots for mutation within MPXV genomes. We found that MPXV genomes were replete with IR sequences. Of the 50+ SNPs identified in the 2022 outbreak strain, 63.9% of these were found to have arisen within IR regions in the 2018 reference strain (MT903344.1). Notably, IR sequences found in the 2018 reference strain were significantly lost over time, with an average of 32.5% of these sequences being conserved in the 2022 MPXV genomes. This evidence was highly indicative that mutations were arising within IRs. This data provides further support to the hypothesis that APOBEC may be driving MPXV mutation and highlights the necessity for greater surveillance of IRs of MPXV genomes to detect new mutations.

Klíčová slova

APOBEC; evolution; inverted repeats; monkeypox; mutation

Autoři

DOBROVOLNÁ, M.; WARNER E. F.; BIDULA S.; BRÁZDA, V.

Vydáno

18. 11. 2022

Nakladatel

WILEY

Místo

HOBOKEN

ISSN

0146-6615

Periodikum

JOURNAL OF MEDICAL VIROLOGY

Ročník

95

Číslo

1

Stát

Spojené státy americké

Strany počet

7

URL

https://onlinelibrary.wiley.com/doi/10.1002/jmv.28322

BibTex

@article{BUT187728,
  author="DOBROVOLNÁ, M. and WARNER E. F. and BIDULA S. and BRÁZDA, V.",
  title="Inverted repeats in the monkeypox virus genome are hot spots for mutation",
  journal="JOURNAL OF MEDICAL VIROLOGY",
  year="2022",
  volume="95",
  number="1",
  pages="7",
  doi="10.1002/jmv.28322",
  issn="0146-6615",
  url="https://onlinelibrary.wiley.com/doi/10.1002/jmv.28322"
}