Detail publikace

An integrated microfluidic platform for nucleic acid testing

SUN, A. VOPAŘILOVÁ, P. LIU, X. KOU, B. ŘEZNÍČEK, T. LEDNICKÝ, T. NI, S. KUDR, J. ZÍTKA, O. FOHLEROVÁ, Z. PAJER, P. ZHANG, H. NEUŽIL, P.

Originální název

An integrated microfluidic platform for nucleic acid testing

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

This study presents a rapid and versatile low-cost sample-to-answer system for SARS-CoV-2 diagnostics. The system integrates the extraction and purification of nucleic acids, followed by amplification via either reverse transcription-quantitative polymerase chain reaction (RT–qPCR) or reverse transcription loop-mediated isothermal amplification (RT–LAMP). By meeting diverse diagnostic and reagent needs, the platform yields testing results that closely align with those of commercial RT-LAMP and RT‒qPCR systems. Notable advantages of our system include its speed and cost-effectiveness. The assay is completed within 28 min, including sample loading (5 min), ribonucleic acid (RNA) extraction (3 min), and RT-LAMP (20 min). The cost of each assay is ≈ $9.5, and this pricing is competitive against that of Food and Drug Administration (FDA)-approved commercial alternatives. Although some RNA loss during on-chip extraction is observed, the platform maintains a potential limit of detection lower than 297 copies. Portability makes the system particularly useful in environments where centralized laboratories are either unavailable or inconveniently located. Another key feature is the platform’s versatility, allowing users to choose between RT‒qPCR or RT‒LAMP tests based on specific requirements.

Klíčová slova

microfluidics;nucleic acids;RT-PCR;SARS-CoV-2

Autoři

SUN, A.; VOPAŘILOVÁ, P.; LIU, X.; KOU, B.; ŘEZNÍČEK, T.; LEDNICKÝ, T.; NI, S.; KUDR, J.; ZÍTKA, O.; FOHLEROVÁ, Z.; PAJER, P.; ZHANG, H.; NEUŽIL, P.

Vydáno

23. 5. 2024

Nakladatel

Nature

ISSN

2055-7434

Periodikum

Microsystems & Nanoengineering

Ročník

10

Číslo

1

Stát

Čínská lidová republika

Strany od

1

Strany do

14

Strany počet

14

URL

https://www.nature.com/articles/s41378-024-00677-6

Plný text v Digitální knihovně

http://hdl.handle.net/11012/249356

BibTex

@article{BUT188684,
  author="Antao {Sun} and Petra {Vopařilová} and Xiaocheng {Liu} and Bingqian {Kou} and Tomáš {Řezníček} and Tomáš {Lednický} and Sheng {Ni} and Jiří {Kudr} and Ondřej {Zítka} and Zdenka {Fohlerová} and Petr {Pajer} and Haoqing {Zhang} and Pavel {Neužil}",
  title="An integrated microfluidic platform for nucleic acid testing",
  journal="Microsystems & Nanoengineering",
  year="2024",
  volume="10",
  number="1",
  pages="1--14",
  doi="10.1038/s41378-024-00677-6",
  issn="2055-7434",
  url="https://www.nature.com/articles/s41378-024-00677-6"
}