Přístupnostní navigace
E-přihláška
Vyhledávání Vyhledat Zavřít
Detail publikace
HEŘMÁNKOVÁ, K. SEDLÁŘ, K.
Originální název
Co-expression analysis of small RNAs and untranslated regions in Rhodospirillum rubrum
Typ
článek ve sborníku mimo WoS a Scopus
Jazyk
angličtina
Originální abstrakt
Bacterial small RNAs (sRNAs) are regulatory elements often mentioned in the context of stress response. So are polyhydroxyalkanoates (PHAs), a possible replacement for conventional plastics, are frequently produced by bacteria facing stress conditions. PHAs then serve as the source of the carbon as well as the defense mechanism against further stress. Here, we present sRNA analysis of PHA-producing bacterium Rhodospirillum rubrum, especially the co-expression analysis of inferred sRNA candidates from RNA-Seq data. Weighted correlation network analysis (WGCNA) was used to compute clusters of genes with similar expression profiles, resulting in 28 modules. Further, the functional annotation of genes in each module was applied and the overall functional profiles were mentioned using a ‘guilt by association’ approach. Additionally, functional enrichment of modules was obtained through over- representation analysis (ORA). These results provide an overview of regulatory elements and their predicted functions in R. rubrum, which further unveil its dynamic properties and can help with the assessment of its industrial application.
Klíčová slova
RNA-Seq; PHA; sRNA; functional annotation; coexpression analysis
Autoři
HEŘMÁNKOVÁ, K.; SEDLÁŘ, K.
Vydáno
24. 4. 2024
ISBN
978-80-214-6231-1
Kniha
Proceedings I of the 30 th Conference STUDENT EEICT 2024
Edice
1
Strany od
113
Strany do
116
Strany počet
4
BibTex
@inproceedings{BUT189094, author="Kristýna {Heřmánková} and Karel {Sedlář}", title="Co-expression analysis of small RNAs and untranslated regions in Rhodospirillum rubrum", booktitle="Proceedings I of the 30 th Conference STUDENT EEICT 2024", year="2024", series="1", pages="113--116", isbn="978-80-214-6231-1" }