Detail publikace

Rapid determination of uracil in biological fluids at mercury thin film electrode for early detection of potential 5-fluorouracil toxicity due to dihydropyrimidine dehydrogenase deficiency

A. M., A. SELCUK, O. MUKHERJEE, A. UNAL, D. KURBANOGLU, S. USLU, B. JUŘICA, J. PEKÁRKOVÁ, J. RICHTERA, L. ADAM, V.

Originální název

Rapid determination of uracil in biological fluids at mercury thin film electrode for early detection of potential 5-fluorouracil toxicity due to dihydropyrimidine dehydrogenase deficiency

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Determination of plasma uracil was reported as a method for evaluation of Dihydropyrimidine dehydrogenase (DPD) activity that is highly demanded to ensure the safe administration of 5-fluorouracil (5-FU)-based therapies to cancer patients. This work reports the development of a simple electroanalytical method based on adsorptive stripping square wave voltammetry (AdSWV) at mercury film-coated glassy carbon electrode (MF/GCE) for the highly sensitive determination of uracil in biological fluids that can be used for diagnosis of decreased DPD activity. Due to the formation of the HgII-Uracil complex at the electrode surface, the accuracy of the measurement was not affected by the complicated matrices in biological fluids including human serum, plasma, and urine. The high sensitivity of the developed method results in a low limit of detection (approximate to 1.3 nM) in human plasma samples, falling below the practical cut-off level of 15 ng mL-1 (approximate to 0.14 mu M). This threshold concentration is crucial for predicting 5-FU toxicity, as reported in buffer, and <= 1.15% in biological samples), and accuracy (recovery percentage close to 100%).

Klíčová slova

Mercury thin film electrode; Adsorptive stripping voltammetry; Uracil determination; Dihydropyrimidine dehydrogenase; phenotyping; 5-Fluorouracil-based therapies

Autoři

A. M., A.; SELCUK, O.; MUKHERJEE, A.; UNAL, D.; KURBANOGLU, S.; USLU, B.; JUŘICA, J.; PEKÁRKOVÁ, J.; RICHTERA, L.; ADAM, V.

Vydáno

28. 6. 2024

Nakladatel

ELSEVIER ADVANCED TECHNOLOGY

Místo

OXFORD

ISSN

1873-4235

Periodikum

BIOSENSORS & BIOELECTRONICS

Ročník

262

Číslo

116545

Stát

Nizozemsko

Strany od

1

Strany do

7

Strany počet

7

URL

https://www.sciencedirect.com/science/article/pii/S0956566324005505?via%3Dihub

BibTex

@article{BUT189918,
  author="Ashrafi {A. M.} and Ozge {Selcuk} and Atripan {Mukherjee} and Didem Nur {Unal} and Sevinc {Kurbanoglu} and Bengi {Uslu} and Jan {Juřica} and Jana {Pekárková} and Lukáš {Richtera} and Vojtěch {Adam}",
  title="Rapid determination of uracil in biological fluids at mercury thin film electrode for early detection of potential 5-fluorouracil toxicity due to dihydropyrimidine dehydrogenase deficiency",
  journal="BIOSENSORS & BIOELECTRONICS",
  year="2024",
  volume="262",
  number="116545",
  pages="7",
  doi="10.1016/j.bios.2024.116545",
  issn="1873-4235",
  url="https://www.sciencedirect.com/science/article/pii/S0956566324005505?via%3Dihub"
}