Detail publikace

Electrochemical and spectrometric study of antioxidant activity of pomiferin, isopomiferin, osajin and catalposide

DIOPAN, V. BABULA, P. SHESTIVSKA, V. ADAM, V. ŽEMLIČKA, M. DVORSKÁ, M. HUBÁLEK, J. TRNKOVÁ, L. HAVEL, L. KIZEK, R.

Originální název

Electrochemical and spectrometric study of antioxidant activity of pomiferin, isopomiferin, osajin and catalposide

Typ

článek v časopise - ostatní, Jost

Jazyk

angličtina

Originální abstrakt

The antioxidant properties of pomiferin, isopomiferin, osajin and catalposide are evaluated. The electrochemical behaviour of these compounds at a carbon paste electrode was studied using square wave voltammetry. Oxidative signals, optimized frequencies and appropriate pH acetate buffer conditionswere determined. The detection limits (3 S/N) for pomiferin, isopomiferin, osajin and catalposide were estimated to be 50 pg/ml, 800 pg/ml, 40 pg/ml and 10 ng/ml, respectively. Furthermore, spectrometric test was employed with 2,2-diphenyl-1-picrylhydrazyle (DPPH) to evaluate the antioxidant activities of these compounds. Based on the obtained results, the highest antioxidant activity measured by DPPH tests was found at pomiferin followed by isopomiferin. The activities of osajin and catalposide were undetectable. The protective effects of pomiferin, isopomiferin, osajin and catalposide on DNA exposed to oxygen radicals in vitrowere also studied.

Klíčová slova

Flavonoids; Iridoid glucoside; DNA; Oxidation signal; Voltammetry; Antioxidant capacity

Autoři

DIOPAN, V.; BABULA, P.; SHESTIVSKA, V.; ADAM, V.; ŽEMLIČKA, M.; DVORSKÁ, M.; HUBÁLEK, J.; TRNKOVÁ, L.; HAVEL, L.; KIZEK, R.

Rok RIV

2008

Vydáno

28. 5. 2008

Nakladatel

ELSEVIER SCIENCE LTD

Místo

OXFORD, ENGLAND

ISSN

0731-7085

Periodikum

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Ročník

2008

Číslo

48

Stát

Nizozemsko

Strany od

127

Strany do

133

Strany počet

7

BibTex

@article{BUT47212,
  author="DIOPAN, V. and BABULA, P. and SHESTIVSKA, V. and ADAM, V. and ŽEMLIČKA, M. and DVORSKÁ, M. and HUBÁLEK, J. and TRNKOVÁ, L. and HAVEL, L. and KIZEK, R.",
  title="Electrochemical and spectrometric study of antioxidant activity of pomiferin, isopomiferin, osajin and catalposide",
  journal="JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS",
  year="2008",
  volume="2008",
  number="48",
  pages="127--133",
  issn="0731-7085"
}