Detail publikace

Monolayers of photosystem II on gold electrodes with enhanced sensor response - effect of porosity and protein layer arrangement

KREJČÍ, J. MALY, J. JAKUBKA, L. ŠTEFFAN, P. MASOJÍDEK, J.

Originální název

Monolayers of photosystem II on gold electrodes with enhanced sensor response - effect of porosity and protein layer arrangement

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Mass transport of the bulk of the analyte to the electrode and through the bioactive layer can be significantly improved by use of the nanoelectrode array and defined arrangement of protein film. This phenomenon has been studied by (i) atomic-force microscopy, (ii) electrochemical measurements of PSII activity, and (iii) digital simulations for an oriented monolayer of histidine-tagged photosystem II (PSII) immobilized on nitrilotriacetic acid (NTA)-modified gold electrodes. The output signal of the electrochemical biosensor is controlled by (i) mass transport from the bioactive layer to electrode and (ii) mass transport between the bulk of the analyte and the electrode. Mass transport through the bioactive layer was electrochemically studied for PSII self-assembled on gold screen-printed electrodes. A densely packed monolayer of PSII has a significant shielding effect toward the diffusion of redox mediator duroquinone (DQ). Mass transport to the planar electrode surface was improved by co-immobilization of bovine-serum albumin (BSA) as spacer biomolecule in the monolayer of PSII. Correlation between the electrochemical properties and surface arrangement of the resulting protein films was clearly observable and confirmed the improved mass-transport properties of structured enzyme monolayers. On the basis of this observation, the application of a bottom-up approach for improvement of electrode performance was proposed and digitally simulated for an infinite array of electrodes ranging in diameter from 50 nm to 5 mu m. The nanoelectrode array, with the optimum time window selected for measurements, enables enhancement of mass transport between the bulk of the analyte and the macroelectrode by a factor of up to 50 in comparison with "classical" planar electrodes. Use of a time window enables minimization of crosstalk between individual electrodes in the array. The measurements require methods which suppress the double-layer capacity.

Klíčová slova

protein monolayer; photosystem II; nanostructured electrodes; biosensors; mass transport

Autoři

KREJČÍ, J.; MALY, J.; JAKUBKA, L.; ŠTEFFAN, P.; MASOJÍDEK, J.

Rok RIV

2005

Vydáno

15. 4. 2005

Nakladatel

SPRINGER HEIDELBERG

Místo

HEIDELBERG, GERMANY

ISSN

1618-2642

Periodikum

Analytical and Bioanalytical Chemistry

Ročník

381

Číslo

8

Stát

Spolková republika Německo

Strany od

1558

Strany do

1567

Strany počet

9

BibTex

@article{BUT48435,
  author="Jan {Krejčí} and Jiri {Maly} and Luboš {Jakubka} and Pavel {Šteffan} and Jiří {Masojídek}",
  title="Monolayers of photosystem II on gold electrodes with enhanced sensor response - effect of porosity and protein layer arrangement",
  journal="Analytical and Bioanalytical Chemistry",
  year="2005",
  volume="381",
  number="8",
  pages="1558--1567",
  issn="1618-2642"
}