Detail publikace

Mathematical evaluation of the amino acid and polyphenol content and antioxidant activities of fruits from different apricot cultivars

SOCHOR, J. ŠKUTKOVÁ, H. BABULA, P. ZÍTKA, O. CERNEI, N. ROP, O. KRŠKA, B. ADAM, V. PROVAZNÍK, I. KIZEK, R.

Originální název

Mathematical evaluation of the amino acid and polyphenol content and antioxidant activities of fruits from different apricot cultivars

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Functional foods are of interest because of their significant effects on human health, which can be connected with the presence of some biologically important compounds. In this study, we carried out complex analysis of 239 apricot cultivars (Prunus armeniaca L.) cultivated in Lednice (climatic area T4), South Moravia, Czech Republic. Almost all previously published studies have focused only on analysis of certain parameters. However, we focused on detection both primary and secondary metabolites in a selection of apricot cultivars with respect to their biological activity. The contents of thirteen biogenic alpha-L-amino acids (arginine, asparagine, isoleucine, lysine, serine, threonine, valine, leucine, phenylalanine, tryptophan, tyrosine, proline and alanine) were determined using ion exchange chromatography with UV-Vis spectrometry detection. Profile of polyphenols, measured as content of ten polyphenols with significant antioxidant properties (gallic acid, procatechinic acid, p-aminobenzoic acid, chlorogenic acid, caffeic acid, vanillin, p-coumaric acid, rutin, ferrulic acid and quercetrin), was determined by high performance liquid chromatography with spectrometric/electrochemical detection. Moreover, content of total phenolics was determined spectrophotometrically using the Folin-Ciocalteu method. Antioxidant activity was determined using five independent spectrophotometric methods: DPPH assay, DMPD method, ABTS method, FRAP and Free Radicals methods. Considering the complexity of the obtained data, they were processed and correlated using bioinformatics techniques (cluster analysis, principal component analysis). The studied apricot cultivars were clustered according to their common biochemical properties, which has not been done before. The observed similarities and differences were discussed.

Klíčová slova

apricot; amino acids; polyphenolics; antioxidant activity; statistical analysis

Autoři

SOCHOR, J.; ŠKUTKOVÁ, H.; BABULA, P.; ZÍTKA, O.; CERNEI, N.; ROP, O.; KRŠKA, B.; ADAM, V.; PROVAZNÍK, I.; KIZEK, R.

Rok RIV

2011

Vydáno

1. 9. 2011

Nakladatel

MDPI

ISSN

1420-3049

Periodikum

MOLECULES

Ročník

16

Číslo

9

Stát

Švýcarská konfederace

Strany od

7428

Strany do

7457

Strany počet

30

URL

https://www.mdpi.com/1420-3049/16/9/7428

Plný text v Digitální knihovně

http://hdl.handle.net/11012/137235

BibTex

@article{BUT73021,
  author="Jiří {Sochor} and Helena {Vítková} and Petr {Babula} and Ondřej {Zítka} and Natalia Vladimirovna {Cernei} and Otakar {Rop} and Boris {Krška} and Vojtěch {Adam} and Valentine {Provazník} and René {Kizek}",
  title="Mathematical evaluation of the amino acid and polyphenol content and antioxidant activities of fruits from different apricot cultivars",
  journal="MOLECULES",
  year="2011",
  volume="16",
  number="9",
  pages="7428--7457",
  doi="10.3390/molecules16097428",
  issn="1420-3049",
  url="https://www.mdpi.com/1420-3049/16/9/7428"
}