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KRAJCAROVÁ, L. NOVOTNÝ, K. BABULA, P. PROVAZNÍK, I. PROCHAZKOVÁ, P. ADAM, V. MARTIN, M. KIZEK, R. KAISER, J.
Originální název
Copper Transport and Accumulation in Spruce Stems (Picea abies (L.) Karsten) Revealed by Laser-Induced Breakdown Spectroscopy
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
Laser-Induced Breakdown Spectroscopy (LIBS) in double pulse configuration (DP LIBS) was used for scanning elemental spatial distribution in annual terminal stems of spruce (Picea abies (L.) Karsten). Cross sections of stems cultivated in Cu2+ solution of different concentrations were prepared and analyzed by DP LIBS. Raster scanning with 150 um spatial resolution was set and 2D (2-dimentional) maps of Cu and Ca distribution were created on the basis of the data obtained. Stem parts originating in the vicinity of the implementation of the cross sections were mineralized and subsequently Cu and Ca contents were analyzed by inductively coupled plasma mass spectrometry (ICP-MS). The results provide quantitative information about overall concentration of the elements in places, where LIBS measurements were performed. The fluorescence pictures were created to compare LIBS distribution maps and the fluorescence intensity (or the increase in autofluorescence) was used for the comparison of ICP-MS quantitative results. Results from these three methods can be utilized for quantitative measurements of copper ions transport in different plant compartments in dependence on the concentration of cultivation medium and/or the time of cultivation.
Klíčová slova
Electrochemical Detection; Double-pulse LIBS; Fluorescence Microscopy; Elemental Distribution; Mass Spectrometry
Autoři
KRAJCAROVÁ, L.; NOVOTNÝ, K.; BABULA, P.; PROVAZNÍK, I.; PROCHAZKOVÁ, P.; ADAM, V.; MARTIN, M.; KIZEK, R.; KAISER, J.
Rok RIV
2013
Vydáno
1. 4. 2013
ISSN
1452-3981
Periodikum
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
Ročník
8
Číslo
4
Stát
Srbská republika
Strany od
4485
Strany do
4504
Strany počet
20
BibTex
@article{BUT99022, author="Lucie {Krajcarová} and Karel {Novotný} and Petr {Babula} and Valentine {Provazník} and Petra {Prochazková} and Vojtěch {Adam} and Madhavi Z. {Martin} and René {Kizek} and Jozef {Kaiser}", title="Copper Transport and Accumulation in Spruce Stems (Picea abies (L.) Karsten) Revealed by Laser-Induced Breakdown Spectroscopy", journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE", year="2013", volume="8", number="4", pages="4485--4504", issn="1452-3981" }