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Project detail
Duration: 01.01.2017 — 31.12.2019
Funding resources
Czech Science Foundation - Standardní projekty
- whole funder (2017-01-20 - 2019-12-31)
On the project
Navrhneme, vyrobíme a ověříme mikrofluidický systém pro mapování vnitřní teploty a monitorování tepelné balance živých buněk. Tento systém bude ve formě mikrokalorimetru s minimálním vnitřním objemem obklopeným vakuem. Vlastní buňky budou v běžném kultivačním médiu při normálním tlaku. Objem mikrokalorimetru řádově ve stovkách femtolitrů v kombinaci s teplotní izolací systému zvýší teplotní změny v buňkách o několik řádů. Pro měření teploty vyvineme různé metody, jako je fluorescence neovlivněná poklesem jasu, teplotně citlivé fluorescenční barvivo a plazmonické teplotně citlivé struktury. Díky speciálnímu pěstování buněk v unikátním mikrokalorimetru budeme schopni reálně rozlišit změny teploty pod 0,1 °C, což je o řád lepší než uvádí dosud publikované práce. Umožní nám to sledovat buněčné reakce v reálném čase, jako je termogeneze v buňkách, mitóza, chemoterapeutikem vyvolaná apoptóza, diferenciace kmenových buněk a energetickou balanci buněk.
Description in EnglishWe will design, fabricate and verify a microfluidic system to map internal temperature and monitor energy balance in living cells. This microcalorimetric (C) system will have minimal volume surrounded with vacuum. Living cells will be inside the C in conventional cell culturing media at normal pressure. The C volume of hundreds of fL in combination with thermal isolation increase the temperature change in cells by several orders of magnitude. We will develop several techniques to measure temperature such as temperature sensitive fluorescent dye independent of a photobleaching effect and temperature sensitive plasmonic structures. Cell culturing in this unique C we will be able to determine temperature with precision better than 0.1 °C, which is one order more sensitive than published works. This sensitivity allows us to monitor internal cell reactions in real time, such as thermogenesis, mitoses, drug induces apoptosis, stem cell differentiation and cell energy balance.
Keywordsmonitorování;vnitřní;teploty;buněk; energetická;balance;buněk; termogenese; MEMS; mikrokalorimetr;
Key words in Englishprecise;temperature;mapping;of;cell;internal;temperature; cell;energy;balance; thermogenesis; MEMS; microcalorimeter;
Mark
GA17-20716S
Default language
Czech
People responsible
Neužil Pavel, prof. Ing., Dr., DSc. - principal person responsible
Units
Smart Nanodevices- beneficiary (2017-01-01 - not assigned)
Results
GABLECH, I.; CAHA, O.; SVATOŠ, V.; PEKÁREK, J.; NEUŽIL, P.; ŠIKOLA, T. Stress-free deposition of [001] preferentially oriented titanium thin film by Kaufman ion-beam source. Thin Solid Films, 2017, vol. 638, no. NA, p. 57-62. ISSN: 0040-6090.Detail
HRDÝ, R.; KYNCLOVÁ, H.; KLEPÁČOVÁ, I.; BARTOŠÍK, M.; NEUŽIL, P. Portable lock-in amplifier-based electrochemical method to measure an array of 64 sensors for point of care applications. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017. 2020. p. 479-480. ISBN: 978-0-69-294183-6.Detail
PODEŠVA, P.; GABLECH, I.; NEUŽIL, P. Nanostructured Gold Microelectrode Array for Ultrasensitive Detection of Heavy Metal Contamination. Analytical Chemistry, 2018, vol. 90, no. 2, p. 1161-1167. ISSN: 1520-6882.Detail
HRDÝ, R.; KYNCLOVÁ, H.; KLEPÁČOVÁ, I.; BARTOŠÍK, M.; NEUŽIL, P. Portable Lock-in Amplifier-Based Electrochemical Method to Measure an Array of 64 Sensors for Point-of-Care Applications. ANALYTICAL CHEMISTRY, 2017, vol. 86, no. 17, p. 8731-8737. ISSN: 0003-2700.Detail
SVATOŠ, V.; GABLECH, I.; PEKÁREK, J.; KLEMPA, J.; NEUŽIL, P. Precise determination of thermal parameters of a microbolometer. INFRARED PHYSICS & TECHNOLOGY, 2018, vol. 93, no. NA, p. 286-290. ISSN: 1350-4495.Detail
FENG, JG; SVATOŠ, V.; LIU, XC.; CHANG, HL.; NEUŽIL, P. High-performance microcalorimeters: Design, applications and future development. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2018, vol. 109, no. NA, p. 43-49. ISSN: 0165-9936.Detail
ZHU, H.; FOHLEROVÁ, Z.; PEKÁREK, J.; BASOVA, E.; NEUŽIL, P. Recent Advances in Lab-on-a-chip Technologies for Viral Diagnosis. BIOSENSORS & BIOELECTRONICS, 2020, no. 1, p. 1-10. ISSN: 0956-5663.Detail
FENG, J.; PODEŠVA, P.; ZHU, H.; PEKÁREK, J.; MAYORGA-MARTINEZ, C.; CHANG, H.; PUMERA, M.; NEUŽIL, P. Droplet-based differential microcalorimeter for real-time energy balance monitoring. Sensors and Actuators B: Chemical, 2020, vol. 312, no. NA, p. 1-7. ISSN: 0925-4005.Detail
NI, S.; ZHU, H.; NEUŽIL, P.; YOBAS, L. A COMPACT MEMS CHIP FOR A RAPID AND HIGHLY ACCURATE PICOLITER CALORIMETRY. In IEEE International Conference on Micro Electro Mechanical Systems. NEW YORK: IEEE, 2020. p. 1006-1009. ISBN: 978-1-7281-3581-6.Detail
Zhu, HL.; Zhang, HQ.; Ni, S.; Korabecna, M.; Yobas, L.; Neuzil, P. The vision of point-of-care PCR tests for the COVID-19 pandemic and beyond. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2020, vol. 130, no. 115984, p. 115984-115984. ISSN: 0165-9936.Detail
Ni, S.; Zhu, HL.; Neuzil, P.; Yobas, L. A SiN Microcalorimeter and a Non-Contact Precision Method of Temperature Calibration. JOURNAL OF MICROELECTROMECHANICAL SYSTEMS, 2020, vol. 29, no. 5, p. 1103-1105. ISSN: 1057-7157.Detail
FENG, J.; ZHU, H.; LUKEŠ, J.; KORABEČNÁ, M.; FOHLEROVÁ, Z.; MEI, T.; CHANG, H.; NEUŽIL, P. Nanowatt simple microcalorimetry for dynamically monitoring the defense mechanism of Paramecium caudatum. Sensors and Actuators, 2021, vol. 323, no. 1, p. 1-9. ISSN: 0924-4247.Detail
NI, S.; BU, Y.; ZHU, H.; NEUŽIL, P.; YOBAS, L. A Sub-nL Chip Calorimeter and Its Application to the Measurement of the Photothermal Transduction Efficiency of Plasmonic Nanoparticles. JOURNAL OF MICROELECTROMECHANICAL SYSTEMS, 2021, vol. 30, no. 5, p. 759-769. ISSN: 1057-7157.Detail
WANG, Y.; ZHU, H.; FENG, J.; NEUŽIL, P. Recent advances of microcalorimetry for studying cellular metabolic heat. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2021, vol. 143, no. 1, p. 116353-1 (116353-10 p.)ISSN: 1879-3142.Detail
GABLECH, I.; CAHA, O.; SVATOŠ, V.; PRÁŠEK, J.; PEKÁREK, J.; NEUŽIL, P.; ŠIKOLA, T. Preparation of [001] Oriented Titanium Thin Film for MEMS Applications by Kaufman Ion-beam Source. In Proceedings of 9 th International conference Nanocon 2017. 1. Ostrava: Tanger, 2018. p. 117-122. ISBN: 978-80-87294-59-8.Detail