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Project detail
Duration: 01.01.2020 — 31.12.2022
Funding resources
Czech Science Foundation - Juniorské granty
- whole funder
On the project
Novel approaches are adapted to fit the histological routine of skin tumors, the most common malignancy. These methods are used in tandem so that they completely characterize biological processes leading to the inhibition and development of lesions in tissues, their type and margins. Spectroscopic techniques are used to investigate changes in the chemical composition of a specimen. Laser-Induced Breakdown Spectroscopy provides a detailed elemental distribution in the full soft tissue section. However, the major issue obstructing its wide exploitation in clinical research is bound to the complex processes beyond the laser ablation of soft tissue and consecutive plasma formation. A thorough understanding of those processes is necessary. In this project, I will investigate parameters influencing the laser-tissue interaction in order to model the processes of laser-induced plasmas (formation and development) with respect to the best analytical performance. Due to the uniqueness of human skin tumors, sections of paraffin-embedded murine (porcine) tissues will serve as model samples.
Keywordslaser-induced plasma;laser ablation;soft tissues;spectroscopy;
Mark
20-19526Y
Default language
English
People responsible
Képeš Erik, Ing., Ph.D. - fellow researcherModlitbová Pavlína, Ing., Ph.D. - fellow researcherVrlíková Lucie, Ing. - fellow researcherPořízka Pavel, doc. Ing., Ph.D. - principal person responsible
Units
Advanced instrumentation and methods for material characterization- beneficiary (2019-03-27 - not assigned)
Results
VRÁBEL, J.; KÉPEŠ, E.; POŘÍZKA, P.; KAISER, J. Distance of spectroscopic data. 2020.Detail
POŘÍZKA, P.; KONEČNÁ, A.; ŠINDELÁŘOVÁ, A.; ŠULCOVÁ, M.; MODLITBOVÁ, P.; PROCHAZKA, D.; NEVORÁNKOVÁ, P.; NAVRÁTIL, M.; VRLÍKOVÁ, L.; BUCHTOVÁ, M.; KAISER, J. Feasibility of laser-induced breakdown spectroscopy to elucidate elemental changes in human tooth ankylosis. SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY, 2023, vol. 206, no. 106727, ISSN: 1873-3565.Detail
BUDAY, J.; ŠINDELÁŘOVÁ, A.; POŘÍZKA, P.; VRLÍKOVÁ, L.; BUCHTOVÁ, M.; KAISER, J. Spectroscopic and morphological information of the laser induced plasma for soft tissues. 2020.Detail
KÉPEŠ, E.; GORNUSHKIN, I.; POŘÍZKA, P.; KAISER, J. Spatial and temporal characterization of double-pulse laser-induced plasmas in the orthogonal geometry. 2020.Detail
POŘÍZKA, P.; ŠINDELÁŘOVÁ, A.; VRLÍKOVÁ, L.; KISS, K.; KAŠKA, M.; BUCHTOVÁ, M.; KAISER, J. Challenges in the laser ablation of soft tissues. Kyoto: 2020. p. 1 (1 s.).Detail
BUDAY, J.; PROCHAZKA, D.; POŘÍZKA, P.; KAISER, J. Computation of blast wave energy in LIBS using Shadowgraphy. INTERNATIONAL WORKSHOP ON LASER-INDUCED BREAKDOWN SPECTROSCOPY. Szeged, Hungary: 2020. p. 90-93. ISBN: 978-963-306-765-9.Detail
ŠINDELÁŘOVÁ, A.; POŘÍZKA, P.; MODLITBOVÁ, P.; VRLÍKOVÁ, L.; KISS, K.; KAŠKA, M.; PROCHAZKA, D.; VRÁBEL, J.; BUCHTOVÁ, M.; KAISER, J. Methodology for the Implementation of Internal Standard to Laser-Induced Breakdown Spectroscopy Analysis of Soft Tissues. SENSORS, 2021, vol. 21, no. 3, p. 1-13. ISSN: 1424-8220.Detail
LIMBECK, A.; BRUNNBAUER, L.; LOHNINGER, H.; POŘÍZKA, P.; MODLITBOVÁ, P.; KAISER, J.; JANOVSZKY, P.; KERI, A.; GALBÁCS, G. Methodology and applications of elemental mapping by laser induced breakdown spectroscopy. Analytica Chimica Acta, 2021, vol. 1147, no. 1, p. 72-98. ISSN: 1873-4324.Detail
POŘÍZKA, P.; VYTISKOVÁ, K.; OBOŘILOVÁ, R.; PASTUCHA, M.; GÁBRIŠ, I.; BRANDMEIER, J.; MODLITBOVÁ, P.; GORRIS, H.; NOVOTNÝ, K.; SKLÁDAL, P.; KAISER, J.; FARKA, Z. Laser-induced breakdown spectroscopy as a readout method for immunocytochemistry with upconversion nanoparticles. Microchimica Acta, 2021, vol. 188, no. 5, p. 147-1 (147-5 p.)ISSN: 0026-3672.Detail
KISS, K.; ŠINDELÁŘOVÁ, A.; KRBAL, L.; STEJSKAL, V.; MRÁZOVÁ, K.; VRÁBEL, J.; KAŠKA, M.; MODLITBOVÁ, P.; POŘÍZKA, P.; KAISER, J. Imaging margins of skin tumors using laser-induced breakdown spectroscopy and machine learning. Journal of Analytical Atomic Spectrometry, 2021, vol. 36, no. 5, p. 909-916. ISSN: 1364-5544.Detail
KAISER, J.; MODLITBOVÁ, P.; PROCHAZKA, D.; POŘÍZKA, P. Benefits of Laser-Induced Breakdown Spectroscopy in large-scale imaging, system development and their use in selected applications. 2021.Detail
BUDAY, J.; POŘÍZKA, P.; KAISER, J.; BUCHTOVÁ, M. Determination of initial expansion energy with shadowgraphy in laser-induced breakdown spectroscopy. Spectrochimica Acta Part B, 2021, vol. 182, no. 106254, p. 106254-1 (106254-6 p.)ISSN: 0584-8547.Detail
KONEČNÁ, A.; POŘÍZKA, P.; MODLITBOVÁ, P.; PROCHAZKA, D.; VRLÍKOVÁ, L.; BUCHTOVÁ, M.; KAISER, J. Víceprvková prostorově rozlišená analýza tvrdých tkání pomocí metody spektroskopie laserem buzeného plazmatu. 2021. ISBN: 978-80-87441-28-2.Detail
KOPŘIVOVÁ, H.; POŘÍZKA, P.; MODLITBOVÁ, P.; PROCHAZKA, D.; VRLÍKOVÁ, L.; BUCHTOVÁ, M.; KAISER, J. Prostorově rozlišená analýza měkkých tkání metodou spektroskopie laserem buzeného plazmatu. 2021. ISBN: 978-80-87441-28-2.Detail
LAVICKÝ, J.; KOLOUŠKOVÁ, M.; PROCHAZKA, D.; RAKULTSEV, V.; GONZALEZ-LOPEZ, M.; STEKLÍKOVÁ, K.; BARTOŠ, M.; VIJAYKUMAR, A.; KAISER, J.; POŘÍZKA, P.; HOVOŘÁKOVÁ, M.; MINA, M.; KŘIVÁNEK, J. The Development of Dentin Microstructure Is Controlled by the Type of Adjacent Epithelium. Journal of Bone and Mineral Research, 2022, vol. 37, no. 2, p. 323-339. ISSN: 1523-4681.Detail
KÉPEŠ, E.; VRÁBEL, J.; POŘÍZKA, P.; KAISER, J. Opening black-box models used in LIBS. 2021.Detail
KÉPEŠ, E.; VRÁBEL, J.; ADAMOVSKÝ, O.; STŘÍTEŽSKÁ, S.; MODLITBOVÁ, P.; POŘÍZKA, P.; KAISER, J. Interpreting support vector machines applied in laser-induced breakdown spectroscopy. Analytica Chimica Acta, 2022, vol. 1192, no. 339352, p. 1-12. ISSN: 1873-4324.Detail
BUDAY, J.; POŘÍZKA, P.; KAISER, J. Využitie Shadowgraphy pre charakterizáciu tlakovej vlny vzniknutej laserovou abláciou materiálu. 2021.Detail
KONEČNÁ, A.; MODLITBOVÁ, P.; PROCHAZKA, D.; KŘIVÁNEK, J.; POŘÍZKA, P.; KAISER, J. Multi-elemental imaging of mouse teeth by Laser-Induced Breakdown Spectroscopy. 2021.Detail
HORÁKOVÁ, K.; MODLITBOVÁ, P.; PROCHAZKA, D.; POŘÍZKA, P.; KAISER, J. Spatially resolved analysis of heavy metals in Cannabis sativa by using LIBS. 2021.Detail
KONEČNÁ, A.; MODLITBOVÁ, P.; PROCHAZKA, D.; KŘIVÁNEK, J.; POŘÍZKA, P.; KAISER, J. Víceprvková prostorově rozlišená analýza myších zubů metodou spektroskopie laserem buzeného plazmatu. 2021.Detail
KOPŘIVOVÁ, H.; POŘÍZKA, P.; MODLITBOVÁ, P.; PROCHAZKA, D.; VRLÍKOVÁ, L.; BUCHTOVÁ, M.; KAISER, J. Imaging of soft tissues by laser-induced breakdown spectroscopy. 2021.Detail
KÉPEŠ, E.; VRÁBEL, J.; POŘÍZKA, P.; KAISER, J. Improving laser-induced breakdown spectroscopy regression models via transfer learning. Journal of Analytical Atomic Spectrometry, 2022, vol. 37, no. 9, p. 1883-1893. ISSN: 1364-5544.Detail
KONEČNÁ, A.; POŘÍZKA, P.; MODLITBOVÁ, P.; PROCHAZKA, D.; VRLÍKOVÁ, L.; BUCHTOVÁ, M.; KAISER, J. Multi-elemental spatially resolved analysis of hard tissues by Laser-Induced Breakdown Spectroscopy. 2022.Detail
KÉPEŠ, E.; VRÁBEL, J.; POŘÍZKA, P.; KAISER, J. Interpreting artificial neural networks used in LIBS. 2022.Detail
KÉPEŠ, E.; VRÁBEL, J.; POŘÍZKA, P.; KAISER, J. Transfer learning for improved LIBS analytical performance. 2022.Detail
POŘÍZKA, P.; BRUNNBAUER, L.; PORKERT, M.; ROZMAN, U.; MAROLT, G.; HOLUB, D.; KIZOVSKÝ, M.; BENEŠOVÁ, M.; SAMEK, O.; LIMBECK, A.; KAISER, J.; KALČÍKOVÁ, G. Laser-based techniques: Novel tools for the identification and characterization of aged microplastics with developed biofilm. CHEMOSPHERE, 2023, vol. 313, no. 1, p. 1-9. ISSN: 0045-6535.Detail
KOPŘIVOVÁ, H.; KISS, K.; BUDAY, J.; VRLÍKOVÁ, L.; KAŠKA, M.; BUCHTOVÁ, M.; KAISER, J.; POŘÍZKA, P. Towards cohort study of cutaneous cancers using laser-induced breakdown spectroscopy. 2022.Detail
KOPŘIVOVÁ, H.; POŘÍZKA, P.; KISS, K.; BUDAY, J.; BRUNNBAUER, L.; KAŠKA, M.; LIMBECK, A.; KAISER, J. Correlative study of cutaneous cancers using LIBS and LA-ICP-MS. 2022.Detail
HOLUB, D.; POŘÍZKA, P.; KIZOVSKÝ, M.; BENEŠOVÁ, M.; KALČÍKOVÁ, G.; SAMEK, O.; KAISER, J. Detection of microplastics in various environments by LIBS and Raman spectroscopy. 2022.Detail
HOLUB, D.; POŘÍZKA, P.; FINTOR, K.; GALBÁCS, G.; KAISER, J. Utilization of Laser Induced Breakdown Spectroscopy and Raman spectroscopy data fusion for classification of polymer materials. 2022.Detail
KÉPEŠ, E.; VRÁBEL, J.; POŘÍZKA, P.; KAISER, J. Transfer learning as a potential tool for improving LIBS analytical performance for space exploration. 2022.Detail
POŘÍZKA, P.; MODLITBOVÁ, P.; KAISER, J. Imaging of Biological Tissues. In Laser-Induced Breakdown Spectroscopy in Biological, Forensic and Materials Sciences. 2022. ISBN: 978-3-031-14502-5.Detail
BUDAY, J.; POŘÍZKA, P.; KAISER, J. Analysis of laser induced plasmas for disparate samples by shadowgraphy. Kyoto: 2020.Detail