Novel approaches are adapted to fit the histological routine of skin tumors, the most common malignancy. These methods are used in tandem so that they completely characterize biological processes leading to the inhibition and development of lesions in tissues, their type and margins. Spectroscopic techniques are used to investigate changes in the chemical composition of a specimen. Laser-Induced Breakdown Spectroscopy provides a detailed elemental distribution in the full soft tissue section. However, the major issue obstructing its wide exploitation in clinical research is bound to the complex processes beyond the laser ablation of soft tissue and consecutive plasma formation. A thorough understanding of those processes is necessary. In this project, I will investigate parameters influencing the laser-tissue interaction in order to model the processes of laser-induced plasmas (formation and development) with respect to the best analytical performance. Due to the uniqueness of human skin tumors, sections of paraffin-embedded murine (porcine) tissues will serve as model samples.

Klíčová slova
laser-induced plasma;laser ablation;soft tissues;spectroscopy;

20-19526Y

angličtina

Pořízka Pavel, doc. Ing., Ph.D. - hlavní řešitel
Képeš Erik, Ing., Ph.D. - spoluřešitel
Modlitbová Pavlína, Ing., Ph.D. - spoluřešitel
Vrlíková Lucie, Ing. - spoluřešitel

Pokročilé instrumentace a metody pro charakterizace materiálů
- odpovědné pracoviště (27.3.2019 - nezadáno)
Pokročilé instrumentace a metody pro charakterizace materiálů
- příjemce (27.3.2019 - nezadáno)

POŘÍZKA, P.; KONEČNÁ, A.; ŠINDELÁŘOVÁ, A.; ŠULCOVÁ, M.; MODLITBOVÁ, P.; PROCHAZKA, D.; NEVORÁNKOVÁ, P.; NAVRÁTIL, M.; VRLÍKOVÁ, L.; BUCHTOVÁ, M.; KAISER, J. Feasibility of laser-induced breakdown spectroscopy to elucidate elemental changes in human tooth ankylosis. SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY, 2023, vol. 206, no. 106727, ISSN: 1873-3565.
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POŘÍZKA, P.; MODLITBOVÁ, P.; MELIKECHI, N.; KAISER, J. Laser-ablation spectroscopy for imaging of tumor markers and nanoparticle labels. In Optical Spectroscopy and Imaging for Cancer Diagnostics: Fundamentals, Progress, and Challenges. World Scientific Pub Co Inc, 2023. p. 181-206. ISBN: 978-981-125-896-1.
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POŘÍZKA, P.; MODLITBOVÁ, P.; KAISER, J. Imaging of Biological Tissues. In Laser-Induced Breakdown Spectroscopy in Biological, Forensic and Materials Sciences. 2022. ISBN: 978-3-031-14502-5.
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KÉPEŠ, E.; VRÁBEL, J.; POŘÍZKA, P.; KAISER, J. Transfer learning as a potential tool for improving LIBS analytical performance for space exploration. 2022.
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HOLUB, D.; POŘÍZKA, P.; FINTOR, K.; GALBÁCS, G.; KAISER, J. Utilization of Laser Induced Breakdown Spectroscopy and Raman spectroscopy data fusion for classification of polymer materials. 2022.
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HOLUB, D.; POŘÍZKA, P.; KIZOVSKÝ, M.; BENEŠOVÁ, M.; KALČÍKOVÁ, G.; SAMEK, O.; KAISER, J. Detection of microplastics in various environments by LIBS and Raman spectroscopy. 2022.
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KOPŘIVOVÁ, H.; POŘÍZKA, P.; KISS, K.; BUDAY, J.; BRUNNBAUER, L.; KAŠKA, M.; LIMBECK, A.; KAISER, J. Correlative study of cutaneous cancers using LIBS and LA-ICP-MS. 2022.
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KOPŘIVOVÁ, H.; KISS, K.; BUDAY, J.; VRLÍKOVÁ, L.; KAŠKA, M.; BUCHTOVÁ, M.; KAISER, J.; POŘÍZKA, P. Towards cohort study of cutaneous cancers using laser-induced breakdown spectroscopy. 2022.
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POŘÍZKA, P.; BRUNNBAUER, L.; PORKERT, M.; ROZMAN, U.; MAROLT, G.; HOLUB, D.; KIZOVSKÝ, M.; BENEŠOVÁ, M.; SAMEK, O.; LIMBECK, A.; KAISER, J.; KALČÍKOVÁ, G. Laser-based techniques: Novel tools for the identification and characterization of aged microplastics with developed biofilm. CHEMOSPHERE, 2023, vol. 313, no. 1, p. 1-9. ISSN: 0045-6535.
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KÉPEŠ, E.; VRÁBEL, J.; POŘÍZKA, P.; KAISER, J. Transfer learning for improved LIBS analytical performance. 2022.
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KÉPEŠ, E.; VRÁBEL, J.; POŘÍZKA, P.; KAISER, J. Interpreting artificial neural networks used in LIBS. 2022.
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KONEČNÁ, A.; POŘÍZKA, P.; MODLITBOVÁ, P.; PROCHAZKA, D.; VRLÍKOVÁ, L.; BUCHTOVÁ, M.; KAISER, J. Multi-elemental spatially resolved analysis of hard tissues by Laser-Induced Breakdown Spectroscopy. 2022.
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KÉPEŠ, E.; VRÁBEL, J.; POŘÍZKA, P.; KAISER, J. Improving laser-induced breakdown spectroscopy regression models via transfer learning. Journal of Analytical Atomic Spectrometry, 2022, vol. 37, no. 9, p. 1883-1893. ISSN: 1364-5544.
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KOPŘIVOVÁ, H.; POŘÍZKA, P.; MODLITBOVÁ, P.; PROCHAZKA, D.; VRLÍKOVÁ, L.; BUCHTOVÁ, M.; KAISER, J. Imaging of soft tissues by laser-induced breakdown spectroscopy. 2021.
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KONEČNÁ, A.; MODLITBOVÁ, P.; PROCHAZKA, D.; KŘIVÁNEK, J.; POŘÍZKA, P.; KAISER, J. Víceprvková prostorově rozlišená analýza myších zubů metodou spektroskopie laserem buzeného plazmatu. 2021.
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HORÁKOVÁ, K.; MODLITBOVÁ, P.; PROCHAZKA, D.; POŘÍZKA, P.; KAISER, J. Spatially resolved analysis of heavy metals in Cannabis sativa by using LIBS. 2021.
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KONEČNÁ, A.; MODLITBOVÁ, P.; PROCHAZKA, D.; KŘIVÁNEK, J.; POŘÍZKA, P.; KAISER, J. Multi-elemental imaging of mouse teeth by Laser-Induced Breakdown Spectroscopy. 2021.
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BUDAY, J.; POŘÍZKA, P.; KAISER, J. Využitie Shadowgraphy pre charakterizáciu tlakovej vlny vzniknutej laserovou abláciou materiálu. 2021.
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KÉPEŠ, E.; VRÁBEL, J.; ADAMOVSKÝ, O.; STŘÍTEŽSKÁ, S.; MODLITBOVÁ, P.; POŘÍZKA, P.; KAISER, J. Interpreting support vector machines applied in laser-induced breakdown spectroscopy. Analytica Chimica Acta, 2022, vol. 1192, no. 339352, p. 1-12. ISSN: 1873-4324.
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KÉPEŠ, E.; VRÁBEL, J.; POŘÍZKA, P.; KAISER, J. Opening black-box models used in LIBS. 2021.
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Odpovědnost: Pořízka Pavel, doc. Ing., Ph.D.