Project detail

Integrated aptamer-nanozyme system for one step label-free voltammetric detection of lung cancer miRNA biomarker

Duration: 01.03.2022 — 28.02.2023

Funding resources

Brno University of Technology - Vnitřní projekty VUT

- whole funder (2022-01-01 - 2023-12-31)

On the project

Gold Nanoparticles (GNPs) with oxidase activity are prepared and fully characterized. The capture probe for the miR-17-3p a lung cancer biomarker is immobilized on the surface of GNPs that deactivates their oxidase activity. In presence of miR-17-3p the capture probe detaches from the surface of the GNPs which restore their oxidase activity and oxidize the TMB substrate. The oxidized TMB gives electrochemical signal that can be used for the sensitive and selective quantification of miR-17-3p.

Mark

CEITEC VUT-J-22-7976

Default language

Czech

People responsible

Richtera Lukáš, doc. RNDr., Ph.D. - fellow researcher
Garehbaghi Sanam, MEng., M.Sc. - principal person responsible

Units

Central European Institute of Technology BUT
- beneficiary (2022-01-01 - 2022-12-31)
Smart Nanodevices
- co-beneficiary (2022-03-01 - 2023-02-28)

Results

GAREHBAGHI, S.; ASHRAFI, A.; ADAM, V.; RICHTERA, L. Surface modification strategies and the functional mechanisms of gold nanozyme in biosensing and bioassay. Materials Today Bio, 2023, vol. 20, no. 1, ISSN: 2590-0064.
Detail

Responsibility: Garehbaghi Sanam, MEng., M.Sc.