Přístupnostní navigace
E-application
Search Search Close
Publication detail
HYNEK, D. TMEJOVÁ, K. TRNKOVÁ, L. HUBÁLEK, J. ADAM, V. KIZEK, R.
Original Title
Automatized determination of metallothionein by adsorptive transfer stripping technique coupled with Brdicka reaction
Type
book chapter
Language
English
Original Abstract
Application of electrochemical methods for protein determination was discovered in the thirties of the last century. Rudolf Brdicka was one who focused his attention to the protein electrochemical determination and used discovered analytical method for clinical purposes. Brdicka way of protein detection was based on the specific reaction between thiol group of proteins with Brdicka solution. Metallothionein (MT) as a low-molecular mass protein with high thiols content seems to be ideal target for such detection. This protein is involved in heavy metals homeostasis due to their chelation centers in MT structure.
Keywords
metallothionein, Brdicka reaction, thiol compound, mercury electrode, tumour diseases, automation
Authors
HYNEK, D.; TMEJOVÁ, K.; TRNKOVÁ, L.; HUBÁLEK, J.; ADAM, V.; KIZEK, R.
RIV year
2013
Released
1. 6. 2013
Publisher
Nova Science Publisher, Inc.
ISBN
978-1-62948-057-2
Book
Voltammetry: Theory, Types and Applications
Edition number
1
Pages from
Pages to
12
Pages count
URL
http://web2.mendelu.cz/af_239_nanotech/data/pub/Kizek%20from%20978-1-62948-057-2.pdf
BibTex
@inbook{BUT101248, author="David {Hynek} and Kateřina {Tmejová} and Libuše {Trnková} and Jaromír {Hubálek} and Vojtěch {Adam} and René {Kizek}", title="Automatized determination of metallothionein by adsorptive transfer stripping technique coupled with Brdicka reaction", booktitle="Voltammetry: Theory, Types and Applications", year="2013", publisher="Nova Science Publisher, Inc.", edition="1", pages="1--12", isbn="978-1-62948-057-2", url="http://web2.mendelu.cz/af_239_nanotech/data/pub/Kizek%20from%20978-1-62948-057-2.pdf" }