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ZÍTKA, O. KŘÍŽKOVÁ, S. SKALIČKOVÁ, S. FIALOVÁ, D. ADAM, V. KIZEK, R.
Original Title
Microfluidic tool coupled with electrochemical assay for detectionof lactoferrin isolated by antibody-modified paramagntetic beads
Type
journal article in Web of Science
Language
English
Original Abstract
Lactoferrin (LF) is approximately 80 kDa iron-binding protein, which is important part of saliva and other body fluids. Due to its ability to bind metal ions, it has many biologically important functions. In this study, a method for the isolation of LF from a biological sample using robotically prepared antibody-modified paramagnetic particles was developed using robotic pipetting station. The method consisted of the following optimised steps. Protein G was bound on the paramagnetic particles, on which goat antibody (10 microg) was linked. LF was subsequently added to microtitration plate, which had affinity to goat antibody and the interaction lasted for 30 min. We found that the highest signals were obtained using the combination of goat antibody 1:3000, murine antibody 1:5000 and conjugate 1:1500
Keywords
Microfluidic tool, elektrochemical detection, lactoferrin
Authors
ZÍTKA, O.; KŘÍŽKOVÁ, S.; SKALIČKOVÁ, S.; FIALOVÁ, D.; ADAM, V.; KIZEK, R.
RIV year
2013
Released
1. 7. 2013
ISBN
0173-0835
Periodical
Electrophoresis
Year of study
34
Number
14
State
Federal Republic of Germany
Pages from
2120
Pages to
2128
Pages count
9
BibTex
@article{BUT103092, author="Ondřej {Zítka} and Soňa {Křížková} and Sylvie {Skaličková} and Dana {Fialová} and Vojtěch {Adam} and René {Kizek}", title="Microfluidic tool coupled with electrochemical assay for detectionof lactoferrin isolated by antibody-modified paramagntetic beads", journal="Electrophoresis", year="2013", volume="34", number="14", pages="2120--2128", doi="10.1002/elps.201200631", issn="0173-0835" }