Publication detail

Microfluidic tool coupled with electrochemical assay for detectionof lactoferrin isolated by antibody-modified paramagntetic beads

ZÍTKA, O. KŘÍŽKOVÁ, S. SKALIČKOVÁ, S. FIALOVÁ, D. ADAM, V. KIZEK, R.

Original Title

Microfluidic tool coupled with electrochemical assay for detectionof lactoferrin isolated by antibody-modified paramagntetic beads

Type

journal article in Web of Science

Language

English

Original Abstract

Lactoferrin (LF) is approximately 80 kDa iron-binding protein, which is important part of saliva and other body fluids. Due to its ability to bind metal ions, it has many biologically important functions. In this study, a method for the isolation of LF from a biological sample using robotically prepared antibody-modified paramagnetic particles was developed using robotic pipetting station. The method consisted of the following optimised steps. Protein G was bound on the paramagnetic particles, on which goat antibody (10 microg) was linked. LF was subsequently added to microtitration plate, which had affinity to goat antibody and the interaction lasted for 30 min. We found that the highest signals were obtained using the combination of goat antibody 1:3000, murine antibody 1:5000 and conjugate 1:1500

Keywords

Microfluidic tool, elektrochemical detection, lactoferrin

Authors

ZÍTKA, O.; KŘÍŽKOVÁ, S.; SKALIČKOVÁ, S.; FIALOVÁ, D.; ADAM, V.; KIZEK, R.

RIV year

2013

Released

1. 7. 2013

ISBN

0173-0835

Periodical

Electrophoresis

Year of study

34

Number

14

State

Federal Republic of Germany

Pages from

2120

Pages to

2128

Pages count

9

BibTex

@article{BUT103092,
  author="Ondřej {Zítka} and Soňa {Křížková} and Sylvie {Skaličková} and Dana {Fialová} and Vojtěch {Adam} and René {Kizek}",
  title="Microfluidic tool coupled with electrochemical assay for detectionof lactoferrin isolated by antibody-modified paramagntetic beads",
  journal="Electrophoresis",
  year="2013",
  volume="34",
  number="14",
  pages="2120--2128",
  doi="10.1002/elps.201200631",
  issn="0173-0835"
}